NLK Antibody (2B11) - Azide and BSA Free

Western Blot: NLK Antibody (2B11) [H00051701-M02] - NLK monoclonal antibody (M02), clone 2B11. Analysis of NLK expression in human liver.

Western Blot: NLK Antibody (2B11) [H00051701-M02] - NLK monoclonal antibody (M02), clone 2B11. Western Blot analysis of NLK expression in PC-12(Cat # L012V1 ).

Western Blot: NLK Antibody (2B11) [H00051701-M02] - NLK monoclonal antibody (M02), clone 2B11 Western Blot analysis of NLK expression in HepG2 ( Cat # L019V1 ).

Western Blot: NLK Antibody (2B11) [H00051701-M02] - NLK monoclonal antibody (M02), clone 2B11. Western Blot analysis of NLK expression in NIH/3T3(Cat # L018V1 ).

Western Blot: NLK Antibody (2B11) [H00051701-M02] - Analysis of NLK expression in transfected 293T cell line by NLK monoclonal antibody (M02), clone 2B11.Lane 1: NLK transfected lysate (Predicted MW: 57.1 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: NLK Antibody (2B11) [H00051701-M02] - Analysis of protein-protein interactions between MAP3K7 and NLK. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-NLK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 2B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NLK Antibody (2B11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NLK Antibody (2B11) - Azide and BSA Free (H00051701-M02) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: NLK (AAH64663, 416 a.a. ~ 515 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE
• Specificity: NLK - nemo-like kinase (2B11)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NLK Antibody (2B11) - Azide and BSA Free

• DKFZp761G1211
• EC 2.7.11
• EC 2.7.11.24
• FLJ21033
• LAK1
• nemo like kinase
• nemo-like kinaseserine/threonine-protein kinase NLK
• Protein LAK1

Background

Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP . Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLK
• Entrez:
  • 51701(Human)
• Uniprot:
  • AAH64663(Human)