NKX6.1 Antibody - BSA Free

NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0017.jpg

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human colon shows no positivity in glandular cells as expected.

NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0018.jpg

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human liver shows no positivity as expected.

Product Discontinued

NKX6.1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PLGTHNPGGLKPPATGGLSSLGSPPQQLSAATPHGINDILSRPSM
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKX6-1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NKX6.1 Antibody - BSA Free

• Homeobox protein NK-6 homolog A
• NK homeo box, family 6, member A
• NK homeobox (Drosophila), family 6, A
• NK homeobox, family 6, A
• NK6 homeobox 1
• NK6 transcription factor homolog A
• NK6 transcription factor related, locus 1 (Drosophila)
• NK6 transcription factor related, locus 1
• NKX6.1
• NKX6-1
• NKX6A
• NKX6Ahomeobox protein Nkx-6.1

Background

In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKX6.1 Antibody (NBP1-82553)(15)

Publications using NBP1-82553

• Hermann FM, Kjaergaard MF, Tian C et al. An insulin hypersecretion phenotype precedes pancreaticb cell failure in MODY3 patient-specific cells Cell stem cell 2022-12-19 [PMID: 36563694]
• Qiao J, Zhang Z, Ji S et Al. A distinct role of STING in regulating glucose homeostasis through insulin sensitivity and insulin secretion Proc Natl Acad Sci U S A 2022-02-10 [PMID: 35145023]
• Maachi H, Ghislain J, Tremblay C et Al. Pronounced proliferation of non-beta cells in response to beta-cell mitogens in isolated human islets of Langerhans Sci Rep 2021-05-28 [PMID: 34050242]
• J Siehler, AK Blöchinger, M Akgün, X Wang, A Shahryari, A Geerlof, H Lickert, I Burtscher Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8) Stem Cell Research, 2020-12-16;50(0):102126. 2020-12-16 [PMID: 33373890]
• Ghila L, Bjorlykke Y, Legoy TA et al. Bioinformatic Analyses of miRNA-mRNA Signature during hiPSC Differentiation towards Insulin-Producing Cells upon HNF4 alpha Mutation Biomedicines 2020-06-27 [PMID: 32605028] (ICC/IF, Human)
• AK Blöchinger, J Siehler, K Wi beta miller, A Shahryari, I Burtscher, H Lickert Generation of an INSULIN-H2B-Cherry reporter human iPSC line Stem Cell Res, 2020-04-22;45(0):101797. 2020-04-22 [PMID: 32361463]
• Legoy TA, Vethe H, Abadpour S et al. Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling Sci Rep 2020-01-15 [PMID: 31942009] (ICC/IF, Human)
• Legoy TA, Ghila L, Vethe H et al. In vivo hyperglycemia exposure elicits distinct period-dependent effects on human pancreatic progenitor differentiation, conveyed by oxidative stress Acta Physiol (Oxf) 2019-12-23 [PMID: 31872528] (IF/IHC, Mouse)
• Wang X, Malinowski AR, Beckenbauer J et al. Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual Stem Cell Res 2019-08-07 [PMID: 31419739] (FLOW, Human)
• Duijkers FA, McDonald A, Janssens GE et al. The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation Front Endocrinol (Lausanne) 2019-05-08 [PMID: 31139151] (ICC/IF, Human)
• Wang X, Sterr M, Ansarullah et al. Point mutations in the PDX1 transactivation domain impair human b-cell development and function Mol Metab 2019-03-20 [PMID: 30930126] (FLOW, ICC/IF, Human)
• Vethe H, Bjorlykke Y, Ghila LM et al. Probing the missing mature B-cell proteomic landscape in differentiating patient iPSC-derived cells Sci Rep 2017-07-06 [PMID: 28684784] (ICC/IF, Human)
• Bader E, Migliorini A, Gegg M et al. Identification of proliferative and mature Beta -cells in the islets of Langerhans Nature 2016-07-21 [PMID: 27398620]
• Pasquali L, Gaulton KJ, Rodriguez-Segui SA et al. Pancreatic islet enhancer clusters enriched in type 2 diabetes risk-associated variants. Nat Genet 2014-02-01 [PMID: 24413736]
• Fiori JL, Shin YK, Kim W et al. Resveratrol Prevents beta-cell Dedifferentiation in Non-Human Primates Given a High Fat/ High Sugar Diet. Diabetes 2013-07-24 [PMID: 23884882]

Bioinformatics

• Gene Symbol: NKX6-1
• Entrez:
  • 4825(Human)
• Uniprot:
  • P78426(Human)