NKX6.1 Antibody (631438) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 631438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

NKX6.1 Antibody (631438) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human NKX6.1
  Met1-Pro120
  Accession # P78426
• Specificity: Detects human NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NKX2E, 2.2, 3.1 or recombinant mouse NKX3.1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX6.1 Antibody (631438) [Alexa Fluor® 532]

• Homeobox protein NK-6 homolog A
• NK homeo box, family 6, member A
• NK homeobox (Drosophila), family 6, A
• NK homeobox, family 6, A
• NK6 homeobox 1
• NK6 transcription factor homolog A
• NK6 transcription factor related, locus 1 (Drosophila)
• NK6 transcription factor related, locus 1
• NKX6.1
• NKX6-1
• NKX6A
• NKX6Ahomeobox protein Nkx-6.1

Background

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces  beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136-173), a DNA binding homeobox (aa 236-295) and a Glu:Asp-rich transactivation domain (aa 305-337). Over aa 1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.