NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 134522
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
• Specificity: Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
  cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]

• CD159 antigen-like family member C
• CD159c antigen
• CD159c
• killer cell lectin-like receptor subfamily C, member 2
• KLRC2
• MGC138244
• NK cell receptor C
• NKG2-C type II integral membrane protein
• NKG2C
• NKG2-C
• NKG2CNKG2-C-activating NK receptor

Background

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8⁺ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8⁺ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C⁺ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). 

1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
6. Braud, V.M. et al. (1998) Nature. 391:795.
7. Lanier, L.L. (1998) Immunity 8:693.
8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
10. Bellón T. et al. (1999) J Immunol 162:3996.
11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2C/CD159c/KLRC2 Antibody (MAB1381)(19)

Publications using MAB1381

• Sánchez-Cerrillo, I;Agudo-Lera, M;Popova, O;Tsukalov, I;Calvet-Mirabent, M;de Los Santos, I;García-Fraile, L;Fuentes, P;Delgado-Arévalo, C;Alcain, J;Sánchez-Gaona, N;Grau-Expósito, J;Lázaro-Díez, M;Muñoz-Calleja, C;Alfranca, A;Genescà, M;Prado, JG;Vrbanac, V;Balazs, A;Buzón, MJ;Toribio, ML;Muñoz-Fernández, MA;Sánchez-Madrid, F;Martín-Gayo, E; Combined dendritic cell and anti-TIGIT immunotherapy potentiates adaptive NK cells against HIV-1 EMBO molecular medicine 2025-06-05 [PMID: 40473838] (Neutralization, Human)
• Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay et al. KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors Frontiers in Immunology 8/21/2023 [PMID: 37675109]
• Y Maeda, H Wada, D Sugiyama, T Saito, T Irie, K Itahashi, K Minoura, S Suzuki, T Kojima, K Kakimi, J Nakajima, T Funakoshi, S Iida, M Oka, T Shimamura, T Doi, Y Doki, E Nakayama, R Ueda, H Nishikawa Depletion of central memory CD8+ T cells might impede the antitumor therapeutic effect of Mogamulizumab Nature Communications, 2021-12-14;12(1):7280. 2021-12-14 [PMID: 34907192] (Neutralization, Human)
• GT Hò, AA Celik, T Huyton, W Hiemisch, R Blasczyk, GS Simper, C Bade-Doedi NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain Int J Mol Sci, 2020-06-19;21(12):. 2020-06-19 [PMID: 32575403] (Affinity Chromatography, ELISA Capture, Human)
• Marcel Costa-García, Michelle Ataya, Manuela Moraru, Carlos Vilches, Miguel López-Botet, Aura Muntasell Human Cytomegalovirus Antigen Presentation by HLA-DR+ NKG2C+ Adaptive NK Cells Specifically Activates Polyfunctional Effector Memory CD4+ T Lymphocytes Frontiers in Immunology 4/3/2019 [PMID: 31001281]
• WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia Int J Mol Sci, 2019-03-22;20(6):. 2019-03-22 [PMID: 30909402] (Immunoprecipitation, Human)
• T Fali, L Papagno, C Bayard, Y Mouloud, J Boddaert, D Sauce, V Appay New Insights into Lymphocyte Differentiation and Aging from Telomere Length and Telomerase Activity Measurements J. Immunol., 2019-02-08;0(0):. 2/8/2019 [PMID: 30737273]
• W Ye, M Chew, J Hou, F Lai, SJ Leopold, HL Loo, A Ghose, AK Dutta, Q Chen, EE Ooi, NJ White, AM Dondorp, P Preiser, J Chen Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway PLoS Pathog., 2018-10-04;14(10):e1007298. 2018-10-04 [PMID: 30286211] (Flow Cytometry, Human)
• CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection Sci Rep, 2017-12-07;7(1):17126. 2017-12-07 [PMID: 29215081] (Flow Cytometry, Marmoset)
• Vassilios Lougaris NFKB1 regulates human NK cell maturation and effector functions Clin. Immunol, 2016-12-03;0(0):. 2016-12-03 [PMID: 27923702] (Flow Cytometry, Human)
• Dolores Redondo-Pa Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients J. Immunol, 2016-12-02;0(0):. 2016-12-02 [PMID: 27913630] (Flow Cytometry, Human)
• Alexander W. Kay, Dara M. Strauss-Albee, Catherine A. Blish Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells Natural Killer Cells 1/25/2017 [PMID: 27177653]
• Anton O, Vielkind S, Peterson M, Tagaya Y, Long E NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors. J Immunol, 2015-10-09;195(10):4810-21. 2015-10-09 [PMID: 26453750] (Flow Cytometry, Human)
• Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. J Immunol, 2015-09-28;195(9):4524-36. 2015-09-28 [PMID: 26416275] (CyTof, Human)
• Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus. J Immunol, 2015-02-09;194(6):2715-24. 2015-02-09 [PMID: 25667418] (Flow Cytometry, Human)
• Heatley , Susan L, Pietra , Gabriell, Lin , Jie, Widjaja , Jacqueli, Harpur , Christop, Lester , Sue, Rossjohn , Jamie, Szer , Jeff, Schwarer , Anthony, Bradstock , Kenneth, Bardy , Peter G, Mingari , Maria Cr, Moretta , Lorenzo, Sullivan , Lucy C, Brooks , Andrew G Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells. J Biol Chem, 2013-01-18;288(12):8679-90. 2013-01-18 [PMID: 23335510] (Flow Cytometry, Human)
• Isabelle Riederer, Wolfgang Sievert, Günther Eissner, Michael Molls, Gabriele Multhoff Irradiation-Induced Up-Regulation of HLA-E on Macrovascular Endothelial Cells Confers Protection against Killing by Activated Natural Killer Cells PLoS ONE 12/16/2010 [PMID: 21179573]
• Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Blood, 2008-01-11;111(6):3155-62. 2008-01-11 [PMID: 18192509] (Neutralization, Human)
• Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007-04-01;109(7):2806-14. 2007-04-01 [PMID: 17179229] (Neutralization, Human)

Review for NKG2C/CD159c/KLRC2 Antibody (MAB1381) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer Flow Other 02/10/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 24660852
• Species: Other

Bioinformatics

• Gene Symbol: KLRC2