NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]

Images

 
Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-reported
Clone
134522
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Specificity
Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
Details of Functionality
ActivityAssaywCitation not found None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KLRC2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-reported
Reviewed Applications
Read 1 Review rated 4
using
MAB1381 in the following applications:

Publications
Read Publications using
MAB1381 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]

  • CD159 antigen-like family member C
  • CD159c antigen
  • CD159c
  • killer cell lectin-like receptor subfamily C, member 2
  • KLRC2
  • MGC138244
  • NK cell receptor C
  • NKG2-C type II integral membrane protein
  • NKG2C
  • NKG2-C
  • NKG2CNKG2-C-activating NK receptor

Background

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).
  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.
  13. Alici, E. et al. (2008) Blood 111:3155.
  14. Coupel, S. et al. (2007) Blood 109:2806.
  15. Fausther-Bovendo, H. et al. (2008) AIDS 22:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2C/CD159c/KLRC2 Antibody (MAB1381)(11)

We have publications tested in 2 confirmed species: Human, Marmoset.

We have publications tested in 4 applications: CyTof, Flow Cytometry, Immunoprecipitation, Neutralization.


Filter By Application
CyTof
(1)
Flow Cytometry
(7)
Immunoprecipitation
(1)
Neutralization
(2)
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Filter By Species
Human
(10)
Marmoset
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB1381 Applications Species
WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30909402] (Immunoprecipitation, Human) Immunoprecipitation Human
W Ye, M Chew, J Hou, F Lai, SJ Leopold, HL Loo, A Ghose, AK Dutta, Q Chen, EE Ooi, NJ White, AM Dondorp, P Preiser, J Chen Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway PLoS Pathog., 2018;14(10):e1007298. 2018 [PMID: 30286211] (Flow Cytometry, Human) Flow Cytometry Human
CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection Sci Rep, 2017;7(1):17126. 2017 [PMID: 29215081] (Flow Cytometry, Marmoset) Flow Cytometry Marmoset
Vassilios Lougaris NFKB1 regulates human NK cell maturation and effector functions Clin. Immunol, 2016;0(0):. 2016 [PMID: 27923702] (Flow Cytometry, Human) Flow Cytometry Human
Dolores Redondo-Pa Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients J. Immunol, 2016;0(0):. 2016 [PMID: 27913630] (Flow Cytometry, Human) Flow Cytometry Human
Anton O, Vielkind S, Peterson M, Tagaya Y, Long E NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors. J Immunol, 2015;195(10):4810-21. 2015 [PMID: 26453750] (Flow Cytometry, Human) Flow Cytometry Human
Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. J Immunol, 2015;195(9):4524-36. 2015 [PMID: 26416275] (CyTof, Human) CyTof Human
Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus. J Immunol, 2015;194(6):2715-24. 2015 [PMID: 25667418] (Flow Cytometry, Human) Flow Cytometry Human
Heatley, Susan L, Pietra, Gabriell, Lin, Jie, Widjaja, Jacqueli, Harpur, Christop, Lester, Sue, Rossjohn, Jamie, Szer, Jeff, Schwarer, Anthony, Bradstock, Kenneth, Bardy, Peter G, Mingari, Maria Cr, Moretta, Lorenzo, Sullivan, Lucy C, Brooks, Andrew G Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells. J Biol Chem, 2013;288(12):8679-90. 2013 [PMID: 23335510] (Flow Cytometry, Human) Flow Cytometry Human
Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Blood, 2008;111(6):3155-62. 2008 [PMID: 18192509] (Neutralization, Human) Neutralization Human
Show All 11 Publications.

Review for NKG2C/CD159c/KLRC2 Antibody (MAB1381) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using MAB1381:
Filter by Applications
Flow
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
Flow Other 02/10/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24660852
SpeciesOther

Blocking

Blocking DetailsSee PMID 24660852

Details

Detection NotesSee PMID 24660852

Comments

CommentsIndependent review from antibodyresource.com

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FAQs for NKG2C/CD159c/KLRC2 Antibody (MAB1381) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1381U
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Alexa Fluor 594 FAB1381T
Alexa Fluor 647 FAB1381R
Alexa Fluor 700 FAB1381N
Alexa Fluor 750 FAB1381S

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Recent Reviews

4
5
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4
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1
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Anonymous
02/10/2015
Application: Flow
Species: Other

Bioinformatics

Gene Symbol KLRC2