NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated] Summary
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
|Details of Functionality
ActivityAssaywCitation not found None
Protein A or G purified from hybridoma culture supernatant
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 0.25 ug/10^6 cells
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]
- CD159 antigen-like family member C
- CD159c antigen
- killer cell lectin-like receptor subfamily C, member 2
- NK cell receptor C
- NKG2-C type II integral membrane protein
- NKG2CNKG2-C-activating NK receptor
Human NKG2C (NK cell Group 2 isoform C; Killer
cell lectin-like receptor subfamily C, member 2) is a member of the C-type
lectin-like superfamily of proteins. Natural
killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+
T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the
activating and inhibitory receptors is important and lack of such regulation
has been implicated in autoimmunity (4).
The NKG2 family includes seven receptors:
NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family
members form heterodimers with CD94 (5, 6).
NKG2C interacts with the adapter molecule DAP12 and acts as activating
receptor when heterodimerized with CD94 (7).
Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa
cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular
domain (ECD). Within the ECD, human NKG2C
shares 40% sequence identity with mouse NKG2C. NKG2C-CD94
heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E
(Qa-1b in mouse), which presents a peptide derived from the signal peptide of
classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may
activate the cytolytic activity and cytokine production of NK and CD8+
(8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation
and expansion of NKG2C+
NK cell subsets, possibly involving a cognate interaction
of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).
- Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
- Tassi I. et al. (2006) Immnunol Rev. 214:92.
- Lanier, L.L. (2008) Nat. Immunol. 9:495.
- Schleinitz, N. et al. (2010) Immunology. 174:2878.
- Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
- Braud, V.M. et al. (1998) Nature. 391:795.
- Lanier, L.L. (1998) Immunity 8:693.
- Vance, R.E. et al. (1999) J Exp Med 190:1801.
- Kaiser B.K. et al. (2005) J Immunol 174:2878.
- Bellón T. et al. (1999) J Immunol 162:3996.
- Pupuleku A. et al. (2017) Front Immunol. 8:1317.
- Hammer Q. et al. (2018) Nat Immunol. 19:453.
- Alici, E. et al. (2008) Blood 111:3155.
- Coupel, S. et al. (2007) Blood 109:2806.
- Fausther-Bovendo, H. et al. (2008) AIDS 22:217.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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