NKAP Antibody

NKAP antibody detects NKAP protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NKAP antibody (NBP3-48584) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

NKAP antibody detects NKAP protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. NKAP antibody (NBP3-48584) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

NKAP antibody detects NKAP protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NKAP Antibody Summary

• Description: Novus Biologicals Rabbit NKAP Antibody (NBP3-48584) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NKAP. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NKAP Antibody

• FLJ22626
• NF-kappaB activating protein
• NF-kappa-B-activating protein
• NFKB activating protein

Background

NKAP is a transcriptional repressor that associates with the NOTCH (see MIM 190198) corepressor complex and is requiredfor T-cell development (Pajerowski et al., 2009 (PubMed 19409814)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKAP