NIK/MAP3K14 Antibody (603917R) [Unconjugated]

NIK/MAP3K14 was detected in immersion fixed paraffin-embedded sections of human pancrease using Mouse Anti-Human NIK/MAP3K14 Monoclonal Antibody (Catalog # MAB6888R) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine and endocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 603917R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NIK/MAP3K14 Antibody (603917R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human NIK/MAP3K14
  Glu769-Pro947
  Accession # Q99558
• Specificity: Detects human NIK/MAP3K14 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NIK/MAP3K14 Antibody (603917R) [Unconjugated]

• EC 2.7.11.25
• FTDCR1B
• HS
• HSNIK
• HSNIKserine/threonine protein-kinase
• MAP3K14
• mitogen-activated protein kinase kinase kinase 14
• NIK
• NIKNF-kappa-beta-inducing kinase
• Serine/threonine-protein kinase NIK

Background

NIK (NF kappa B inducing kinase), also called MAP3K14, is a widely expressed 947 amino acid (aa), ~100 kDa cytoplasmic protein of the MAP kinase family. NIK participates in a non-canonical NFkB signaling cascade in which its activity and expression are elevated by TNF and related ligands. NIK activates IKKa/b, which releases NFkB subunits for translocation to the nucleus. Within the region used as an immunogen, human NIK shares 94% and 95% aa sequence identity with mouse and rat NIK, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99558()