Nicastrin Antibody (716918) [Alexa Fluor® 532]

• Applications: ICC/IF
• Clone: 716918
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Nicastrin Antibody (716918) [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin
  Asn34-Glu669
  Accession # Q92542
• Specificity: Detects human Nicastrin in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Nicastrin (NCT) is a 150‑160 kDa member of the Nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as Notch-1 and APP. Mature human Nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.