Recombinant Human NHERF-1 GST (N-Term) Protein

SDS-Page: Recombinant Human NHERF-1 Protein [H00009368-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human NHERF-1 GST (N-Term) Protein Summary

• Description: A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 252-358 of Human NHERF-1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: NGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: SLC9A3R1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 37.51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human NHERF-1 GST (N-Term) Protein

• EBP50NHERF-1
• ezrin-radixin-moesin binding phosphoprotein-50
• Ezrin-radixin-moesin-binding phosphoprotein 50
• Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Na+/H+ exchange regulatory co-factor
• NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
• NHERFNPHLOP2
• Regulatory cofactor of Na(+)/H(+) exchanger
• sodium/hydrogen exchanger regulatory factor 1
• Sodium-hydrogen exchanger regulatory factor 1
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Solute carrier family 9 isoform A3 regulatory factor 1

Background

Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A3R1