NHERF-1 Antibody - Azide and BSA Free

Product Discontinued

NHERF-1 Antibody - Azide and BSA Free Summary

• Immunogen: The immunogen for this antibody was a sequence from 240a.a. to 358a.a. from EBP50(a 358a.a. long protein)
• Localization: Apical Membrane Protein
• Specificity: NHERF-1
• Clonality: Polyclonal
• Host: Chicken
• Gene: SLC9A3R1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:200-1:500
• Application Notes: ELISA: Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody.
• Control:

  NHERF-1 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for NHERF-1 Antibody - Azide and BSA Free

• EBP50NHERF-1
• ezrin-radixin-moesin binding phosphoprotein-50
• Ezrin-radixin-moesin-binding phosphoprotein 50
• Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Na+/H+ exchange regulatory co-factor
• NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
• NHERFNPHLOP2
• Regulatory cofactor of Na(+)/H(+) exchanger
• sodium/hydrogen exchanger regulatory factor 1
• Sodium-hydrogen exchanger regulatory factor 1
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Solute carrier family 9 isoform A3 regulatory factor 1

Background

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHERF-1 Antibody (NB100-75340)(1)

Publication using NB100-75340

• Heng S, Cervero A, Simon C et al. Proprotein Convertase 5/6 Is Critical for Embryo Implantation in Women: Regulating Receptivity by Cleaving EBP50, Modulating Ezrin Binding, and Membrane-Cytoskeletal Interactions. Endocrinology. 2011-10-04 [PMID: 21971156]

Review for NHERF-1 Antibody (NB100-75340) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 05/09/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: A431 human epithelial carcinoma cell line
• Species: Human
• Lot: 039k2277v

Bioinformatics

• Gene Symbol: SLC9A3R1
• Entrez:
  • 9368(Human)
• Uniprot:
  • O14745(Human)