NHERF-1 Antibody

Images

 
Western Blot: Placenta protein extract as test antigen. Affinity-purified IgY dilution: 1:500, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SLC9A3R1 )
ELISA: NHERF-1 Antibody [NB100-75340] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NHERF-1 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 240a.a. to 358a.a. from EBP50(a 358a.a. long protein)
Localization
Apical Membrane Protein
Specificity
NHERF-1
Clonality
Polyclonal
Host
Chicken
Gene
SLC9A3R1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
ELISA: Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody.
Positive Control
NHERF-1 Lysate (NBL1-16198)
Reviewed Applications
Read 1 Review rated 4
using
NB100-75340 in the following applications:

Publications
Read Publication using NB100-75340.

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NHERF-1 Antibody

  • EBP50NHERF-1
  • ezrin-radixin-moesin binding phosphoprotein-50
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF1
  • Na+/H+ exchange regulatory co-factor
  • NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • NHERFNPHLOP2
  • Regulatory cofactor of Na(+)/H(+) exchanger
  • sodium/hydrogen exchanger regulatory factor 1
  • Sodium-hydrogen exchanger regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Solute carrier family 9 isoform A3 regulatory factor 1

Background

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NHERF-1 Antibody (NB100-75340)(1)

Review for NHERF-1 Antibody (NB100-75340) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NB100-75340:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry NHERF-1 NB100-75340
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reviewed by:
Anonymous
ICC Human 05/09/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedA431 human epithelial carcinoma cell line
SpeciesHuman
Lot039k2277v

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Anonymous
05/09/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SLC9A3R1
Entrez
Uniprot