NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - A: mouse liver 7.5% SDS PAGE diluted at 1:1000

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody. A: Non-transfected lysate. B: NR4A1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] -Untreated and treated Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human NGFI-B alpha/Nur77/NR4A1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (94%), Canine (96%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR4A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

• Early response protein NAK1
• GFRP1ST-59
• growth factor-inducible nuclear protein N10
• HMR
• HMRNP10
• hormone receptor
• MGC9485
• N10
• NAK1
• NAK-1
• NGFIB alpha
• NGFI-B alpha
• NGFIB
• NR4A1
• Nuclear hormone receptor NUR/77
• nuclear receptor subfamily 4 group A member 1
• nuclear receptor subfamily 4, group A, member 1
• Nur77
• Orphan nuclear receptor HMR
• Orphan nuclear receptor TR3
• steroid receptor TR3
• Testicular receptor 3
• TR3 orphan receptor
• TR3

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NR4A1
• Uniprot:
  • P22736(Human)