NGEF Antibody

Western Blot: NGEF Antibody [NBP3-23338] - Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-NGEF (C-terminal region) (lanes 1 & 2) and anti-NGEF (Tyr-87) (lanes 3 & 4).

Immunocytochemistry/Immunofluorescence: NGEF Antibody [NBP3-23338] - Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal NGEF (C-terminal region) and NGEF (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NGEF Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: NGEF synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse NGEF. This sequence has significant homology to the same region in human and rat NGEF, and has low homology to other known proteins. [UniProt# Q8CHT1]
• Specificity: The antibody detects an 80 kDa band corresponding to NGEF in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NGEF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for NGEF Antibody

• EPHEXIN
• ephexin-1
• Eph-interacting exchange protein
• neuronal guanine nucleotide exchange factorARHGEF27

Background

NGEF acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity swi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NGEF