NGDN Overexpression Lysate (Native)

Images

 
Western Blot: NGDN Overexpression Lysate (Adult Normal) [NBL1-13632] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NGDN.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NGDN Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204792 and based on accession number NM_001042635. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens neuroguidin, EIF4E binding protein (NGDN), transcript variant 1, mRNA.
Gene
NGDN

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NGDN

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NGDN Overexpression Lysate (Native)

  • C14orf120
  • DKFZP564O092
  • EIF4E-binding protein
  • eukaryotic initiation factor 4E and CPEB binding protein
  • LCP5
  • lpd-2
  • neuroguidin
  • neuroguidin, EIF4E binding protein
  • NGDchromosome 14 open reading frame 120

Background

Neuroguidin is an EIF4E (MIM 133440)-binding protein that interacts with CPEB (MIM 607342) and functions as a translational regulatory protein during development of the vertebrate nervous system (Jung et al., 2006 [PubMed 16705177]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NGDN