NFYB Antibody (6H6)

Images

 
Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: ...read more
Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Sandwich ELISA: NFYB Antibody (6H6) [H00004801-M01] - Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, RNAi
Clone
6H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NFYB Antibody (6H6) Summary

Immunogen
NFYB (AAH05317, 1 a.a. - 207 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Specificity
NFYB - nuclear transcription factor Y, beta
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFYB
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • RNA Inhibition
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFYB Antibody (6H6)

  • CAAT box DNA-binding protein subunit B
  • CBF-A
  • HAP3CBF-B
  • NF-YBCCAAT-binding transcription factor subunit A
  • Nuclear transcription factor Y subunit B
  • nuclear transcription factor Y subunit beta
  • nuclear transcription factor Y, beta
  • Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFYB
Entrez
OMIM
Uniprot