NFkB p100/p52 Antibody

Product Discontinued

NFkB p100/p52 Antibody Summary

• Description: This antibody was prepared from monospecific antiserum by delipidation and defibrination.
• Immunogen: Human NFkB p100/p52 peptide corresponding to aa residue 1-19 the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKB2
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:25000
  • Western Blot 1:500 - 1:3000
• Application Notes: This product was assayed by immunoblot and found to be reactive against Human NFKB2 p52 at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti- Human NFKB2 p52 is suitable for the detection by immunoblot of Human NFKB2 p52 and its precursor protein p100. Cross reactivity with p52 from other species may occur but has not been specifically determined. Reactivity in supershift assays has not been determined. This product was assayed by immunoblot and found to be reactive against Human NFKB2 p52 at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti- Human NFKB2 p52 is suitable for the detection by immunoblot of Human NFKB2 p52 and its precursor protein p100. Cross reactivity with p52 from other species may occur but has not been specifically determined. Reactivity in supershift assays has not been determined. This product was assayed by immunoblot and found to be reactive against Human NFKB2 p52 at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti- Human NFKB2 p52 is suitable for the detection by immunoblot of Human NFKB2 p52 and its precursor protein p100. Cross reactivity with p52 from other species may occur but has not been specifically determined. Reactivity in supershift assays has not been determined.
• Control Peptide:

  NFkB p100/p52 Protein (NBP2-22372PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for NFkB p100/p52 Antibody

• DNA-binding factor KBF2
• H2TF1
• Lymphocyte translocation chromosome 10 protein
• Lyt10
• LYT10LYT-10
• nuclear factor NF-kappa-B p100 subunit
• nuclear factor of kappa light chain gene enhancer in B-cells 2
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
• Oncogene Lyt-10
• p52

Background

NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by an inhibitor called IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKB2
• Uniprot:
  • Q00653()