NFIC Antibody - BSA Free

Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.

Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NFIC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NFIC Antibody - BSA Free (NBP1-71879) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 250 and 300 of human Nuclear Factor I/C using the numbering given in entry EAW69324.1 (GeneID 4782).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFIC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFIC Antibody - BSA Free

• CCAAT-binding transcription factor
• CTF5
• CTFCCAAT-box-binding transcription factor
• MGC20153
• NF1-C
• NFI
• NF-I
• NF-I/C
• NFI-C
• nuclear factor 1 C-type
• Nuclear factor 1/C
• nuclear factor I/C (CCAAT-binding transcription factor)
• Nuclear factor I/C
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NFIC Antibody (NBP1-71879). (Showing 1 - 2 of 2 FAQ).

1. I am interested to purchase an NFI-C specific antibody. Has it been tested to see if it cross-reacts with other NFI members?
   • Unfortunately, we have not tested against other NFI members.
2. Can you tell me if this antibody against NFI-C NBP1-71879 would be suitable for detecting NFI-C in mice.
   • This Ab should be suitable for detecting NFI-C in mice based on the % homology between the mouse and human protein which is 96.0%. (Our lab recommends use if the % is 85% or above.) As we have not tested this Ab in mice samples you would be eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: NFIC
• Uniprot:
  • EAW69324.1(Human)