NFIB Antibodies
We offer NFIB Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each NFIB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NFIB Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the NFIB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NFIB polyclonal antibodies and browse our NFIB monoclonal antibody catalog.
Alternate Names for NFIB Antibodies
anti-NFIB antibody, anti-CCAAT-box-binding transcription factor antibody, anti-CTF antibody, anti-HMGIC/NFIB antibody, anti-NF-I/B antibody, anti-NFI-B antibody, anti-NFI-RED antibody, anti-NFIB2 antibody, anti-NFIB3 antibody, anti-nuclear factor 1 B-type antibody, anti-Nuclear factor 1/B antibody, anti-nuclear factor I/BNF1-B antibody, anti-TGGCA-binding protein antibody
10 Results for "nfib" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81000-img0009.jpg "Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.")
![Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/NFIB-Antibody-Western-Blot-NBP1-81000-img0007.jpg)
![Western Blot: NFIB Antibody (2D6) [H00004781-M08] - Analysis of NFIB expression in transfected 293T cell line by NFIB monoclonal antibody (M08), clone 2D6. Lane 1: NFIB transfected lysatE (47.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIB-Antibody-2D6-Western-Blot-H00004781-M08-img0005.jpg "Western Blot: NFIB Antibody (2D6) [H00004781-M08] - Analysis of NFIB expression in transfected 293T cell line by NFIB monoclonal antibody (M08), clone 2D6. Lane 1: NFIB transfected lysatE (47.4 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NFIB-Antibody-2D6-Immunocytochemistry-Immunofluorescence-H00004781-M08-img0003.jpg)
![Immunohistochemistry-Paraffin: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/NFIB-Antibody-2D6-Immunohistochemistry-Paraffin-H00004781-M08-img0004.jpg)
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Western-Blot-NBP2-19526-img0002.jpg "Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526] - Paraffin-embedded mouse liver. NFIB antibody [N1C2] dilution: 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunohistochemistry-Paraffin-NBP2-19526-img0004.jpg)
![Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526] - Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunohistochemistry-Paraffin-NBP2-19526-img0003.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIB antibody [N1C2] (NBP2-19526) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19526_rabbit-polyclonal-nfib-antibody-9102024209148.jpg)
![Immunoprecipitation: NFIB Antibody [NBP2-04121] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunoprecipitation-NBP2-04121-img0002.jpg "Immunoprecipitation: NFIB Antibody [NBP2-04121] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![Immunoprecipitation: NFIB Antibody [NBP2-04122] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunoprecipitation-NBP2-04122-img0001.jpg "Immunoprecipitation: NFIB Antibody [NBP2-04122] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![Western Blot: NFIB Antibody (7T1G5) [NBP3-16757] - Western blot analysis of extracts of various cell lines, using NFIB antibody (NBP3-16757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/NFIB-Antibody-7T1G5-Western-Blot-NBP3-16757-img0001.jpg "Western Blot: NFIB Antibody (7T1G5) [NBP3-16757] - Western blot analysis of extracts of various cell lines, using NFIB antibody (NBP3-16757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: NFIB Antibody (S03-0E5) [NBP3-19716] - Western blot detection of NFIB in C6, Hela cell lysates using NBP3-19716 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/NFIB-Antibody-S03-0E5-Western-Blot-NBP3-19716-img0001.jpg "Western Blot: NFIB Antibody (S03-0E5) [NBP3-19716] - Western blot detection of NFIB in C6, Hela cell lysates using NBP3-19716 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")