NFIB Antibody (JE35-21)

Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JE35-21
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NFIB Antibody (JE35-21) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human NFIB aa 371-420 / 420. (Uniprot: O00712)
• Localization: Nucleus
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFIB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:5000-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NFIB Antibody (JE35-21)

• CCAAT-box-binding transcription factor
• CTF
• HMGIC/NFIB
• NF-I/B
• NFI-B
• NFIB2
• NFIB3
• NFI-RED
• nuclear factor 1 B-type
• Nuclear factor 1/B
• nuclear factor I/BNF1-B
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIB