NFATC3/NFAT4 Overexpression Lysate

Images

 
Western Blot: NFAT4 Overexpression Lysate (Adult Normal) [NBL1-13611] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFAT4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NFATC3/NFAT4 Overexpression Lysate Summary

Description

NFATC3/NFAT4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200357

Accession#: NM_173165

Protein Tag: C-MYC/DDK
Gene
NFATC3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
115.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NFATC3/NFAT4 Overexpression Lysate

  • NFAT4
  • NF-AT4
  • NFAT4nuclear factor of activated T-cells, cytoplasmic 3
  • NFATC3
  • NF-ATc3
  • NFATX
  • nuclear factor of activated T-cells c3 isoform IE-Xa
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
  • T cell transcription factor NFAT4
  • T-cell transcription factor NFAT4

Background

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NFATC3