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NFATC3/NFAT4 Antibody [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-99332AF350
Alexa Fluor 405
NBP2-99332AF405
Alexa Fluor 488
NBP2-99332AF488
Alexa Fluor 532
NBP2-99332AF532
Alexa Fluor 594
NBP2-99332AF594
Alexa Fluor 647
NBP2-99332AF647
Alexa Fluor 700
NBP2-99332AF700
Alexa Fluor 750
NBP2-99332AF750
Biotin
NBP2-99332B
DyLight 350
NBP2-99332UV
DyLight 405
NBP2-99332V
DyLight 488
NBP2-99332G
DyLight 550
NBP2-99332R
DyLight 594
NBP2-99332DL594
DyLight 680
NBP2-99332FR
DyLight 755
NBP2-99332IR
FITC
NBP2-99332F
HRP
NBP2-99332H
Janelia Fluor 549
NBP2-99332JF549
Janelia Fluor 646
NBP2-99332JF646
Unconjugated
NBP2-99332-100ul
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NFATC3/NFAT4 Antibody [DyLight 650] Summary

Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NFATC3/NFAT4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFATC3
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NFATC3/NFAT4 Antibody [DyLight 650]

  • NFAT4
  • NF-AT4
  • NFAT4nuclear factor of activated T-cells, cytoplasmic 3
  • NFATC3
  • NF-ATc3
  • NFATX
  • nuclear factor of activated T-cells c3 isoform IE-Xa
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
  • T cell transcription factor NFAT4
  • T-cell transcription factor NFAT4

Background

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in Hela Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in MCF-7 Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.
Simple Western: NFATC1/NFAT2 Antibody [NB100-56732] - Lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Immunohistochemistry-Paraffin: NFATC4 Antibody [NBP1-46210] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NFAT3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: NFATC4 Antibody [NBP1-46210] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NFAT3 antibody NBP1-46211 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFAT3 was also immunoprecipitated by rabbit anti-NFAT3 antibody A302-770A, which recognizes a downstream epitope.
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MAB8930
Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 μg/mL of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c‑Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c‑Junphosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-HumanPhospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-c‑Jun (S63) at approximately 49 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
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Complement C3
Antibody (11H9) - BSA ...
NB200-540
Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.
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(2H2)
NBP2-50037
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
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(228601) [Unconjugate
MAB17761
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(28 Publications)
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Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NFATC3/NFAT4
Antibody [DyLight 650
NBP2-99332C
NFATC3/NFAT4 Antibody [DyLight 650]
Species: Hu
Applications: WB, ICC/IF

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WB Video Protocol
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFATC3