NFATC3/NFAT4 Antibody (646115)

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Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
646115
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

NFATC3/NFAT4 Antibody (646115) Summary

Immunogen
E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968
Specificity
Detects human NFATC3 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NFATC3
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB5834 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFATC3/NFAT4 Antibody (646115)

  • NFAT4
  • NF-AT4
  • NFAT4nuclear factor of activated T-cells, cytoplasmic 3
  • NFATC3
  • NF-ATc3
  • NFATX
  • nuclear factor of activated T-cells c3 isoform IE-Xa
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
  • T cell transcription factor NFAT4
  • T-cell transcription factor NFAT4

Background

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two NLSs (aa 273-275 and 686‑688), one NES (aa 1032-1041) and an RHD that binds DNA (aa 433-592). There are multiple isoforms. Thirty amino acid, 33 aa and 10 aa substitutions for aa 1036‑1075 are known that may be accompanied by an alternate start site at Met480. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFATC3/NFAT4 Antibody (MAB5834)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Filter By Species
Human
(1)
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Bioinformatics

Gene Symbol NFATC3
Uniprot