NFATC3/NFAT4 Antibody (646115)

Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human NFATC3 Monoclonal Antibody (Catalog # MAB5834) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 646115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NFATC3/NFAT4 Antibody (646115) Summary

• Immunogen: E. coli-derived recombinant human NFATC3
  Asp900-Asp1035
  Accession # Q12968
• Specificity: Detects human NFATC3 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFATC3
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFATC3/NFAT4 Antibody (646115)

• NFAT4
• NF-AT4
• NFAT4nuclear factor of activated T-cells, cytoplasmic 3
• NFATC3
• NF-ATc3
• NFATX
• nuclear factor of activated T-cells c3 isoform IE-Xa
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• T cell transcription factor NFAT4
• T-cell transcription factor NFAT4

Background

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4⁺ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca⁺2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two NLSs (aa 273-275 and 686‑688), one NES (aa 1032-1041) and an RHD that binds DNA (aa 433-592). There are multiple isoforms. Thirty amino acid, 33 aa and 10 aa substitutions for aa 1036‑1075 are known that may be accompanied by an alternate start site at Met480. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFATC3/NFAT4 Antibody (MAB5834)(1)

Publication using MAB5834

• J Bond, C Tran Quang, G Hypolite, M Belhocine, A Bergon, G Cordonnier, J Ghysdael, E Macintyre, N Boissel, S Spicuglia, V Asnafi Novel Intergenically-spliced Chimera, NFATC3-PLA2G15, is Associated with Aggressive T-ALL Biology and Outcome Mol. Cancer Res., 2018;0(0):. 2018 [PMID: 29330284] (ICC, Human)

Bioinformatics

• Gene Symbol: NFATC3
• Uniprot:
  • Q12968()