NFATC1/NFAT2 Antibody (7A6)

Images

 
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human spleen tissue.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunohistochemistry: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human tonsil tissue.
Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human skeletal muscle tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Pm, RbSpecies Glossary
Applications WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP, ICC/IF
Clone
7A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NFATC1/NFAT2 Antibody (7A6) Summary

Immunogen
Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
Specificity
Detects cytosolic nuclear factor of activated T-cells (NFAT 2) from transfected human, mouse, and monkey cells. This does not cross react with NFATp.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFATC1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 1:250
  • Immunofluorescence 1:10 - 1:100
Application Notes
WB: Detects an approx. 105 kDa band representing transfected NFAT2 from COS cell lysate.
Control
NFATC1/NFAT2 Overexpression Lysate
NFATC1/NFAT2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB300-620 in the following applications:

Publications
Read Publication using
NB300-620 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16129432). Hamster reactivity reported in scientific literature (PMID: 10866678). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for NFATC1/NFAT2 Antibody (7A6)

  • MGC138448
  • NFAT transcription complex cytosolic component
  • NFAT2
  • NFAT2cytoplasmic 1
  • NFATC
  • NF-ATC
  • NFATC1
  • NF-ATc1
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56508
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-504
Species: Hu, Mu, Rt
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
6507-IL/CF
Species: Hu
H00001513-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-56715
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-45293
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, PAGE, IF
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
NBP2-77240
Species: Hu
H00057410-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF5834
Species: Hu, Mu, Rt
Applications: WB
NB300-620
Species: Hu, Mu, Rt, Ha, Pm, Rb
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP, ICC/IF

Publications for NFATC1/NFAT2 Antibody (NB300-620)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(1)
IHC
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for NFATC1/NFAT2 Antibody (NB300-620) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB300-620:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NFATC1/NFAT2 NB300-620
Enlarge
reviewed by:
Anonymous
WB Mouse 11/10/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse beta cell line
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for NFATC1/NFAT2 Antibody (NB300-620). (Showing 1 - 1 of 1 FAQ).

  1. You list ChIP as an application for this ab (NB300-620). Do you have any data to support it? Which species?
    • The collaborator we use to produce this monoclonal antibody has validated its use in ChIP via several publications. We do not list them on our datasheets as the products were not obtained directly through us. I am sorry for any confusion. I can provide the listings for you, please see the following links (Human: PMID 15347678 and PMID 19564342), (Mouse: PMID 12065418, PMID 15229217 and PMID 22490438), (Rat: PMID 18667424 and PMID 22566696). As ChIP is listed as a verified application on our datasheet, we will fully guarantee its function for this antibody.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional NFATC1/NFAT2 Products

Bioinformatics Tool for NFATC1/NFAT2 Antibody (NB300-620)

Discover related pathways, diseases and genes to NFATC1/NFAT2 Antibody (NB300-620). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NFATC1/NFAT2 Antibody (NB300-620)

Discover more about diseases related to NFATC1/NFAT2 Antibody (NB300-620).
 

Pathways for NFATC1/NFAT2 Antibody (NB300-620)

View related products by pathway.

PTMs for NFATC1/NFAT2 Antibody (NB300-620)

Learn more about PTMs related to NFATC1/NFAT2 Antibody (NB300-620).
 

Research Areas for NFATC1/NFAT2 Antibody (NB300-620)

Find related products by research area.

Blogs on NFATC1/NFAT2

There are no specific blogs for NFATC1/NFAT2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Anonymous
11/10/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol NFATC1
Entrez
Uniprot