Neuropilin-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Neuropilin‑2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Poly­clonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Neuropilin-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
Gln23-Tyr855
Accession # Q7LBX6
Specificity
Detects human, mouse, and rat Neuropilin-2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NRP2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Publications
Read Publications using
AF2215 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-2 Antibody [Unconjugated]

  • MGC126574
  • neuropilin 2
  • Neuropilin2
  • Neuropilin-2
  • neuropilin-2a(22)
  • neuropilin-2b(0)
  • NP2
  • NPN 2
  • NPN2
  • PRO2714
  • receptor for VEGF165 and semaphorins class3
  • Vascular endothelial cell growth factor 165 receptor 2
  • vascular endothelial growth factor-165 receptor 2
  • VEGF1265R2
  • VEGF165R2neuropilin-2a(17)

Background

Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor now known as Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (meprin/A-5 protein/tyrosine phosphatase μ) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2, and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGF R1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

  1. Chen, H. et al. (1997) Neuron 19:547.
  2. Rossignol, M. et al. (2000) Genomics 70:211.
  3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
  4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
  5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuropilin-2 Antibody (AF2215)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Blocking, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
ELISA Development
(1)
Flow Cytometry
(2)
ICC
(3)
IHC
(2)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(12)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF2215 Applications Species
SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism Elife, 2019;8(0):. 2019 [PMID: 31291191] (IHC-Fr, Human) IHC-Fr Human
Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cell, 0;174(5):1158-1171.e19. 0 [PMID: 30057110] (Western Blot, Human) Western Blot Human
M Raaben, LT Jae, AS Herbert, AI Kuehne, SH Stubbs, YY Chou, VA Blomen, T Kirchhause, JM Dye, TR Brummelkam, SP Whelan NRP2 and CD63 Are Host Factors for Lujo Virus Cell Entry Cell Host Microbe, 2017;22(5):688-696.e5. 2017 [PMID: 29120745] (Flow Cytometry, Human) Flow Cytometry Human
LD Volk-Drape, KL Hall, AC Wilber, S Ran Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4 PLoS ONE, 2017;12(6):e0179257. 2017 [PMID: 28598999] (ICC, Human) ICC Human
Promotion of metastasis of thyroid cancer cells via NRP-2-mediated induction Oncol Lett, 2016;12(5):4224-4230. 2016 [PMID: 27895796] (Neutralization, Human) Neutralization Human
Class 3 semaphorins induce F-actin reorganization in human dendritic cells: Role in cell migration J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27406993] (Flow Cytometry, Human) Flow Cytometry Human
Dutta S, Roy S, Polavaram N, Stanton M, Zhang H, Bhola T, Honscheid P, Donohue T, Band H, Batra S, Muders M, Datta K Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology. Cancer Res, 2016;76(2):418-28. 2016 [PMID: 26560516] (Western Blot, Human) Western Blot Human
Wang, Hong-Bo, Zhang, Hua, Zhang, Jing-Pin, Li, Yan, Zhao, Bo, Feng, Guo-Kai, Du, Yong, Xiong, Dan, Zhong, Qian, Liu, Wan-Li, Du, Huamao, Li, Man-Zhi, Huang, Wen-Lin, Tsao, Sai Wah, Hutt-Fletcher, Lindsey, Zeng, Yi-Xin, Kieff, Elliott, Zeng, Mu-Sheng Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells. Nat Commun, 2015;6(0):6240. 2015 [PMID: 25670642] (Blocking, Human) Blocking Human
Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer. Oncoimmunology, 2014;3(0):e29080. 2014 [PMID: 25101222] (ICC, Human) ICC Human
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014;9(1):e84179. 2014 [PMID: 24392113] (ICC, Human) ICC Human
Show All 14 Publications.

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Bioinformatics

Gene Symbol NRP2
Uniprot