Novus Biologicals products are now on

Neuropilin-2 Antibody [Unconjugated]


Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Neuropilin-2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2215) at more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 more
HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Neuropillin-2 Polyclonal Antibody (Catalog # AF2215, filled histogram) or Goat IgG control antibody (AB-108-C, open histogram), more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Neuropilin-2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
Accession # Q7LBX6
Detects human, mouse, and rat Neuropilin-2 in Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Read Publications using
AF2215 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-2 Antibody [Unconjugated]

  • MGC126574
  • neuropilin 2
  • Neuropilin2
  • Neuropilin-2
  • neuropilin-2a(22)
  • neuropilin-2b(0)
  • NP2
  • NPN 2
  • NPN2
  • PRO2714
  • receptor for VEGF165 and semaphorins class3
  • Vascular endothelial cell growth factor 165 receptor 2
  • vascular endothelial growth factor-165 receptor 2
  • VEGF1265R2
  • VEGF165R2neuropilin-2a(17)


Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor now known as Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (meprin/A-5 protein/tyrosine phosphatase μ) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2, and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGF R1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

  1. Chen, H. et al. (1997) Neuron 19:547.
  2. Rossignol, M. et al. (2000) Genomics 70:211.
  3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
  4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
  5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
Species: Hu
Applications: ELISA
Species: Mu
Applications: BA
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: BA, BA
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Neut, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Species: Bv, Ca, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Mu
Applications: BA
Species: Mu
Applications: CyTOF-ready, Flow, WB
Species: Mu
Applications: IHC, WB
Species: Mu, Rt
Applications: IHC, WB

Publications for Neuropilin-2 Antibody (AF2215)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 8 applications: Blocking, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization, Western Blot.

Filter By Application
ELISA Development
Flow Cytometry
Western Blot
All Applications
Filter By Species
Transgenic Mouse
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using AF2215 Applications Species
Hung, YH;Hou, YC;Hsu, SH;Wang, LY;Tsai, YL;Shan, YS;Su, YY;Hung, WC;Chen, LT; Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination American journal of cancer research 2023-08-15 [PMID: 37693128] (Neutralization, Mouse, Human) Neutralization Mouse, Human
Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity Neuron 12/21/2022 [PMID: 36240771]
JA Stogsdill, K Kim, L Binan, SL Farhi, JZ Levin, P Arlotta Pyramidal neuron subtype diversity governs microglia states in the neocortex Nature, 2022-08-10;0(0):. 2022-08-10 [PMID: 35948630] (IHC, Mouse) IHC Mouse
R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance Oncogene, 2022-07-19;40(3):111097. 2022-07-19 [PMID: 35858551] (Western Blot, Human) Western Blot Human
MS Aigrot, C Barthelemy, S Moyon, G Dufayet-Ch, L Izagirre-U, B Gillet-Leg, S Tada, L Bayón-Cord, JC Chara, C Matute, N Cartier, C Lubetzki, V Tepav?evi? Genetically modified macrophages accelerate myelin repair Embo Molecular Medicine, 2022-07-13;0(0):e14759. 2022-07-13 [PMID: 35822550] (Neutralization, Human, Mouse) Neutralization Human, Mouse
S Dutta, NS Polavaram, R Islam, S Bhattachar, S Bodas, T Mayr, S Roy, SAY Albala, MI Toma, A Darehshour, A Borkowetz, S Conrad, S Fuessel, M Wirth, GB Baretton, LC Hofbauer, P Ghosh, KJ Pienta, DL Klinkebiel, SK Batra, MH Muders, K Datta Neuropilin-2 regulates androgen-receptor transcriptional activity in advanced prostate cancer Oncogene, 2022-06-27;0(0):. 2022-06-27 [PMID: 35754042] (Western Blot, Human) Western Blot Human
M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri Structural basis for HCMV Pentamer receptor recognition and antibody neutralization Science Advances, 2022-03-11;8(10):eabm2536. 2022-03-11 [PMID: 35275719] (Flow Cytometry, Human) Flow Cytometry Human
M Aoki, I Gamayun, A Wyatt, R Grünewald, M Simon-Thom, SE Philipp, O Hummel, S Wagenpfeil, K Kattler, G Gasparoni, J Walter, S Qiao, DR Grattan, U Boehm Prolactin-sensitive olfactory sensory neurons regulate male preference in female mice by modulating responses to chemosensory cues Science Advances, 2021-10-08;7(41):eabg4074. 2021-10-08 [PMID: 34623921] (IHC, Mouse) IHC Mouse
MG Dallinga, YI Habani, AWM Schimmel, GM Dallinga-T, CJF van Noorde, I Klaassen, RO Schlingema The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro Cells, 2021-04-16;10(4):. 2021-04-16 [PMID: 33923753] (IHC, Human) IHC Human
Simon Bernatz, Daniel Monden, Florian Gessler, Tijana Radic, Elke Hattingen, Christian Senft et al. Influence of VEGF-A, VEGFR-1-3, and neuropilin 1-2 on progression-free: and overall survival in WHO grade II and III meningioma patients Journal of Molecular Histology 4/1/2021 [PMID: 33528717]
Show All 32 Publications.

Reviews for Neuropilin-2 Antibody (AF2215) (0)

There are no reviews for Neuropilin-2 Antibody (AF2215). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neuropilin-2 Antibody (AF2215) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Neuropilin-2 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol NRP2