Neuropilin-2 Antibody [Unconjugated]


Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Neuropilin‑2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Poly­clonal Antibody (Catalog # more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready

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Neuropilin-2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
Accession # Q7LBX6
Detects human, mouse, and rat Neuropilin-2 in Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Read Publications using
AF2215 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-2 Antibody [Unconjugated]

  • MGC126574
  • neuropilin 2
  • Neuropilin2
  • Neuropilin-2
  • neuropilin-2a(22)
  • neuropilin-2b(0)
  • NP2
  • NPN 2
  • NPN2
  • PRO2714
  • receptor for VEGF165 and semaphorins class3
  • Vascular endothelial cell growth factor 165 receptor 2
  • vascular endothelial growth factor-165 receptor 2
  • VEGF1265R2
  • VEGF165R2neuropilin-2a(17)


Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor now known as Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (meprin/A-5 protein/tyrosine phosphatase μ) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2, and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGF R1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

  1. Chen, H. et al. (1997) Neuron 19:547.
  2. Rossignol, M. et al. (2000) Genomics 70:211.
  3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
  4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
  5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuropilin-2 Antibody (AF2215)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Blocking, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization, Western Blot.

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ELISA Development
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF2215 Applications Species
M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri Structural basis for HCMV Pentamer receptor recognition and antibody neutralization Science Advances, 2022;8(10):eabm2536. 2022 [PMID: 35275719] (Flow Cytometry, Human) Flow Cytometry Human
M Aoki, I Gamayun, A Wyatt, R Grünewald, M Simon-Thom, SE Philipp, O Hummel, S Wagenpfeil, K Kattler, G Gasparoni, J Walter, S Qiao, DR Grattan, U Boehm Prolactin-sensitive olfactory sensory neurons regulate male preference in female mice by modulating responses to chemosensory cues Science Advances, 2021;7(41):eabg4074. 2021 [PMID: 34623921] (IHC, Mouse) IHC Mouse
MG Dallinga, YI Habani, AWM Schimmel, GM Dallinga-T, CJF van Noorde, I Klaassen, RO Schlingema The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro Cells, 2021;10(4):. 2021 [PMID: 33923753] (IHC, Human) IHC Human
G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021;34(4):108644. 2021 [PMID: 33503438] (Neutralization, Mouse) Neutralization Mouse
Y Do, JG Cho, JY Park, S Oh, D Park, KH Yoo, MS Lee, BS Kwon, J Kim, Y Yang MiR-146a Regulates Migration and Invasion by Targeting NRP2 in Circulating-Tumor Cell Mimicking Suspension Cells Genes, 2020;12(1):. 2020 [PMID: 33396906] (Western Blot, Human) Western Blot Human
N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (IHC, Mouse) IHC Mouse
KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32182215] (IHC, Mouse) IHC Mouse
C Lund, V Yellapraga, S Vuoristo, D Balboa, S Trova, C Allet, N Eskici, K Pulli, P Giacobini, T Tuuri, T Raivio Characterization of the human GnRH neuron developmental transcriptome using a GNRH1-TdTomato reporter line in human pluripotent stem cells Dis Model Mech, 2020;0(0):. 2020 [PMID: 31996360] (IHC, Human) IHC Human
SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism Elife, 2019;8(0):. 2019 [PMID: 31291191] (IHC-Fr, Human) IHC-Fr Human
Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cell, 0;174(5):1158-1171.e19. 0 [PMID: 30057110] (Western Blot, Human) Western Blot Human
Show All 22 Publications.

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Gene Symbol NRP2