Neurophysin II/Arg-vasopressin Antibody (62865) [Unconjugated]

Neurophysin II was detected in immersion fixed paraffin-embedded sections of human brain hippocampus tissue using Mouse Anti-Human Neurophysin II Monoclonal Antibody (Catalog # MAB009) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 62865
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Neurophysin II/Arg-vasopressin Antibody (62865) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human Neurophysin II
  Accession # P01186
• Specificity: Detects human Neurophysin II in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurophysin II/Arg-vasopressin Antibody (62865) [Unconjugated]

• arginine vasopressin
• ARVP
• AVP
• AVP-NPII
• AVRP
• neurohypophyseal
• Neurophysin II
• NP2
• vasopressin-neurophysin 2-copeptin
• VP

Background

Arginine vasopressin (AVP)/Neurophysin 2 is a hormone synthesized in neurons in the hypothalamus that specifically binds vasopressin.  Vasopressin has a direct antidiuretic action on the kidney and causes vasoconstriction of the peripheral vessels.  Vasopressin regulates the tonicity of body fluid by causing the kidneys to reabsorb solute-free water and returning it to the circulation from the tubules of the nephron.  AVP released in high concentrations may also raise blood pressure by inducing vasoconstriction.  The stimulus for secretion of vasopressin is increased osmolality of the plasma which is monitored by the hypothalamus.  Vasopressin is often used to manage anti-diuretic hormone deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01186()