Neurolysin Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Neurolysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurolysin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurolysin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Neurolysin
  aa 38-704
• Specificity: Detects human, mouse, and rat Neurolysin Neurolysin in Western blots and human Neurolysin in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Meprin alpha , rhMeprin beta subunit, and rhTHOP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NLN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurolysin Antibody [Unconjugated]

• AGTBP
• AGTBPFLJ23002
• angiotensin binding protein
• Angiotensin-binding protein
• DKFZp564F123
• EC 3.4.24
• EC 3.4.24.16
• KIAA1226EP24.16
• MEP
• mitochondrial
• MOP
• neurolysin (metallopeptidase M3 family)
• Neurolysin
• Neurotensin Endopeptidase
• NLN
• Oligopeptidase M

Background

Neurolysin, also known as Oligopeptidase M (mitochondrial peptidase), soluble angiotensin II-binding protein and endopeptidase EC 3.4.24.16 (1, 2), is a homologue of Thimet Ologopeptidase (THOP1), a zinc peptidase of the M3 family that also includes mitochondrial intermediate peptidase. Neurolysin expresses in two forms with the difference of an N-terminal transit peptide that targets the localization to mitochondria (1). The shorter form exists in cytosol. The recombinant human Neurolysin is expressed without the transit peptide and is isolated from the cytosol. Like THOP1, Neurolysin is capable of cleaving a number of vasoactive peptides such as bradykinin and neurotensin (3). All known substrates of THOP1 and Neurolysin contain 17 or fewer amino acids.

1. Serizawa, A. et al. (1995) J. Biol. Chem. 270:2092.
2. Barett, A.J. and J.M. Chen (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 356, Elsevier Academic Press, San Diego.
3. Norman, M.U. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLN