Neuroligin 1/NLGN1 Antibody

Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neuroligin 1/NLGN1 Antibody Summary

• Immunogen: Peptide with sequence C-TAKQDDPKQQ corresponding to internal region according to NP_055747.1.
• Marker: post-Synaptic Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NLGN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments gave an approx. 150 kDa band in human brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa band according to NP_055747.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Recombinant Human TRIM15 GST (N-Term) Protein
  Recombinant Human TRIM15 GST (N-Term) Protein

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neuroligin 1/NLGN1 Antibody

• EC 3.1.1
• EC 3.1.1.1
• KIAA1070neuroligin-1
• MGC45115
• Neuroligin 1
• NL1
• Nlg
• NLGN1

Background

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLGN1