Neurogenin-3 Antibody (258602) [Unconjugated]

Western blot shows lysates of human heart tissue, human placenta tissue, human prostate tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Neurogenin-3 Monoclonal Antibody (Catalog # MAB3444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neurogenin-3 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 258602
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurogenin-3 Antibody (258602) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Neurogenin-3
  Met1-Leu214
  Accession # Q9Y4Z2
• Specificity: Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Neurogenin-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEUROG3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-3 Antibody (258602) [Unconjugated]

• Atoh5
• BHLHA7
• bHLHa7NGN-3
• Class A basic helix-loop-helix protein 7
• Math4B
• NEUROG3
• neurogenin 3
• Neurogenin3
• Neurogenin-3
• NGN3
• Protein atonal homolog 5
• Relax

Background

Neurogenin-3 (NGN3) is a 23 kDa developmentally expressed nuclear protein of the basic helix-loop-helix (bHLH) family, class B subfamily. bHLH proteins are transcription factors that form dimers to bind DNA. Neurogenin-3 expression is required for all endocrine lineage differentiation in the pancreas, including formation of islet beta  cells. Neurogenin-3 is also neurogenic, controlling the number of dendrites developed by hippocampal neurons. Human Neurogenin-3 shows 76% and 77% amino acid identity with mouse and rat Neurogenin-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEUROG3
• Uniprot:
  • Q9Y4Z2()