Neuregulin-3/NRG3 Antibody (6V9X1)

Western Blot: Neuregulin-3/NRG3 Antibody (6V9X1) [NBP3-15901] - Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3/NRG3) antibody (NBP3-15901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western Blot: Neuregulin-3/NRG3 Antibody (6V9X1) [NBP3-15901] - Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3/NRG3) antibody (NBP3-15901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 6V9X1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Neuregulin-3/NRG3 Antibody (6V9X1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-720 of human Neuregulin-3 (Neuregulin-3/NRG3) (P56975). IIPSVGLEETCLQMPGISEVKSIKWCKNSYSADVVNVSIPVSDCLIAEQQEVKILLETVQEQIRILTDARRSEDYELASVETEDSASENTAFLPLSPTAKSEREAQFVLRNEIQRDSALTK
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NRG3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Neuregulin-3/NRG3 Antibody (6V9X1)

• HRG3
• neuregulin 3 variant 1
• neuregulin 3 variant 10
• neuregulin 3 variant 11
• neuregulin 3 variant 12
• neuregulin 3 variant 13
• neuregulin 3 variant 14
• neuregulin 3 variant 3
• neuregulin 3 variant 4
• neuregulin 3 variant 5
• neuregulin 3 variant 6
• neuregulin 3 variant 7
• neuregulin 3 variant 8
• neuregulin 3 variant 9
• neuregulin 3
• Neuregulin3
• Neuregulin-3
• neuregulin-3-like polypeptide
• NRG3
• pro-neuregulin-3, membrane-bound isoform
• pro-NRG3

Background

NRG3, which belongs to the neuregulin family, is a direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. NRG3 does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. The protein exists as an type I membrane protein and as a proteolytically released soluble growth factor form. The membrane-bound form does not appear to be active. NRG3 is highly expressed in most regions of the brain with the exception of corpus callosum, and is expressed at lower level in testis. It is not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. The NRG3 cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing may involve initial intracellular domain dimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRG3