Home » A2BP1 » A2BP1 Research Products » A2BP1 Antibody (D8H8)

A2BP1 Antibody (D8H8)

 (3 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.
Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.
Simple Western: A2BP1 Antibody (D8H8) [NBP2-13169] - Simple Western lane view shows a specific band for A2BP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
D8H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-13169AF350
Alexa Fluor 405
NBP2-13169AF405
Alexa Fluor 488
NBP2-13169AF488
Alexa Fluor 532
NBP2-13169AF532
Alexa Fluor 594
NBP2-13169AF594
Alexa Fluor 647
NBP2-13169AF647
Alexa Fluor 700
NBP2-13169AF700
Alexa Fluor 750
NBP2-13169AF750
Biotin
NBP2-13169B
DyLight 350
NBP2-13169UV
DyLight 405
NBP2-13169V
DyLight 488
NBP2-13169G
DyLight 550
NBP2-13169R
DyLight 594
NBP2-13169DL594
DyLight 650
NBP2-13169C
DyLight 680
NBP2-13169FR
DyLight 755
NBP2-13169IR
FITC
NBP2-13169F
HRP
NBP2-13169H
Janelia Fluor 549
NBP2-13169JF549
Janelia Fluor 646
NBP2-13169JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80553
100% Guarantee on every product

A2BP1 Antibody (D8H8) Summary

Immunogen
Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
Localization
Nucleus. Cytoplasm. Note: Predominantly nuclear.
Specificity
Does not cross react with paralogue FOX2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RBFOX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
Application Notes
This A2BP1 Antibody (D8H8) is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry on paraffin embedded sections. In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30001398).
Publications
Read Publications using
NBP2-13169 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for A2BP1 Antibody (D8H8)

  • 2BP1
  • A2BP
  • A2BP1 Hexaribonucleotide-binding protein 1
  • Ataxin-2-binding protein 1
  • FOX1 hexaribonucleotide binding protein 1
  • Fox-1 homolog A
  • FOX-1
  • HRNBP1 ataxin 2-binding protein 1
  • RNA binding protein fox-1 homolog 1
  • RNA binding protein, fox-1 homolog (C. elegans) 1

Background

A2BP1, also known as Ataxin-2-binding protein 1, Fox-1 homolog A and Rbfox1, is a member of the Fox-1 family, which is made up of RNA-binding proteins. A2BP1 regulates tissue-specific alternative splicing during erythropoiesis by locating the UGCAUG sequence and binding to the 5'-splice site while also preventing U2AF2 from binding to the 3' splicing site. A2BP1 may play a role in restricting the pathology of spinocerebellar ataxia type 2 (SCA2). The SCA2 gene produces ataxin-2 proteins which is a cause of familial neurodegenerative diseases. A2BP1 is primarily found in the nucleus but also seen in the cytoplasm and is expressed in the brain, heart, neurons, skeletal muscle, and embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FOX2 Antibody
NB110-40588
Western Blot: FOX2 Antibody [NB110-40588] - Detection of mouse and rat RBM9 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - Sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 RBM9 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(7 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Ataxin-2 Antibody
NBP1-90063
Western Blot: Ataxin-2 Antibody [NBP1-90063] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP1-90063] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
PTBP2 Antibody
NBP2-19997
Western Blot: PTBP2 Antibody [NBP2-19997] - A. 30 ug H1299 whole cell lysate/extract B. 30 ug HCT116 whole cell lysate/extract 7.5 % SDS-PAGE PTBP2 antibody [C1C3] dilution: 1:1000
Immunohistochemistry-Paraffin: PTBP2 Antibody [NBP2-19997] - Paraffin-embedded mouse middle brain. PTBP2 antibody [C1C3] dilution: 1:1000.
Western Blot: PTBP2 Antibody [NBP2-19997] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Fibronectin
Antibody
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
(11 Reviews)
(30 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
PTBP1 Antibody
(3H8)
H00005725-M01
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
(5 Publications)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Ly-6E Antibody
NBP1-52176
Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Ly-6E Antibody [NBP1-52176] - Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Mu
Applications: Flow, ICC/IF, PEP-ELISA
Procalcitonin
Antibody (44D9)
NB120-14817
Procalcitonin Antibody (44D9)
(1 Review)
(6 Publications)
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, S-ELISA
Human
Ceruloplasmin ELISA K ...
NBP2-60616
ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616]
Species: Hu
Splicing Factor 1
Antibody (2E12)
H00007536-M01
Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
hnRNP AB Antibody
NBP2-16865
Western Blot: hnRNP AB Antibody [NBP2-16865] - Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: hnRNP AB Antibody [NBP2-16865] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A/B protein stained by hnRNP A/B antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP2-16865] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
HNF-3 beta/FoxA2
Antibody [Unconjugate ...
AF2400
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(38 Publications)
Species: Hu
Applications: WB, IHC, ChIP, ICC
EN-RAGE/S100A12
Antibody [Unconjugate
AF1052
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Reviews)
(2 Publications)
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

SF-1/NR5A1/Steroidogen ...
NBP1-52823
Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.
(3 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Arylsulfatase D
Antibody
NBP1-87486
Western Blot: Arylsulfatase D Antibody [NBP1-87486] - Analysis in control (vector only transfected HEK293T lysate) and ARSD over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Arylsulfatase D Antibody [NBP1-87486] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Arylsulfatase D Antibody [NBP1-87486] - Staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.
Species: Hu
Applications: WB, IHC, IHC-P
DDX39 Antibody
NBP2-16134
Western Blot: DDX39 Antibody [NBP2-16134] - analysis of DDX39 in 293T cell lysate (30ug) using anti-DDX39 antibody. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: DDX39 Antibody [NBP2-16134] - Human lung cancer. DDX39 antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: DDX39 Antibody [NBP2-16134] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
(1 Review)
Species: Hu
Applications: WB, IHC, IHC-P, IP
A2BP1 Antibody
(D8H8)
NBP2-13169
A2BP1 Antibody (D8H8)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for A2BP1 Antibody (NBP2-13169)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-Fr, PCR.


Filter By Application
IHC-Fr
(1)
PCR
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for A2BP1 Antibody (NBP2-13169) (0)

There are no reviews for A2BP1 Antibody (NBP2-13169). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for A2BP1 Antibody (NBP2-13169): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for A2BP1 Antibody (NBP2-13169) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-13169AF350
Alexa Fluor 405 NBP2-13169AF405
Alexa Fluor 488 NBP2-13169AF488
Alexa Fluor 532 NBP2-13169AF532
Alexa Fluor 594 NBP2-13169AF594
Alexa Fluor 647 NBP2-13169AF647
Alexa Fluor 700 NBP2-13169AF700
Alexa Fluor 750 NBP2-13169AF750
Biotin NBP2-13169B
DyLight 350 NBP2-13169UV
DyLight 405 NBP2-13169V
DyLight 488 NBP2-13169G
DyLight 550 NBP2-13169R
DyLight 594 NBP2-13169DL594
DyLight 650 NBP2-13169C
DyLight 680 NBP2-13169FR
DyLight 755 NBP2-13169IR
FITC NBP2-13169F
HRP NBP2-13169H
Janelia Fluor 549 NBP2-13169JF549
Janelia Fluor 646 NBP2-13169JF646
Learn about Custom Antibody Labeling

Additional A2BP1 Products

Bioinformatics Tool for A2BP1 Antibody (NBP2-13169)

Discover related pathways, diseases and genes to A2BP1 Antibody (NBP2-13169). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for A2BP1 Antibody (NBP2-13169)

Discover more about diseases related to A2BP1 Antibody (NBP2-13169).
 

Pathways for A2BP1 Antibody (NBP2-13169)

View related products by pathway.

Research Areas for A2BP1 Antibody (NBP2-13169)

Find related products by research area.

Blogs on A2BP1

There are no specific blogs for A2BP1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  Western Blot Protocol
  ICC/IF Protocol
  IHC-P Protocol
  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our A2BP1 Antibody (D8H8) and receive a gift card or discount.


Bioinformatics

Gene Symbol RBFOX1
Entrez
Uniprot