A2BP1 Antibody (D8H8)

Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.

Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.

Simple Western: A2BP1 Antibody (D8H8) [NBP2-13169] - Simple Western lane view shows a specific band for A2BP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: D8H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

A2BP1 Antibody (D8H8) Summary

• Immunogen: Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
• Localization: Nucleus. Cytoplasm. Note: Predominantly nuclear.
• Specificity: Does not cross react with paralogue FOX2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBFOX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
• Application Notes: This A2BP1 Antibody (D8H8) is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry on paraffin embedded sections. In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30001398).

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine (pH 7.5) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for A2BP1 Antibody (D8H8)

• 2BP1
• A2BP
• A2BP1 Hexaribonucleotide-binding protein 1
• Ataxin-2-binding protein 1
• FOX1 hexaribonucleotide binding protein 1
• Fox-1 homolog A
• FOX-1
• HRNBP1 ataxin 2-binding protein 1
• RNA binding protein fox-1 homolog 1
• RNA binding protein, fox-1 homolog (C. elegans) 1

Background

A2BP1, also known as Ataxin-2-binding protein 1, Fox-1 homolog A and Rbfox1, is a member of the Fox-1 family, which is made up of RNA-binding proteins. A2BP1 regulates tissue-specific alternative splicing during erythropoiesis by locating the UGCAUG sequence and binding to the 5'-splice site while also preventing U2AF2 from binding to the 3' splicing site. A2BP1 may play a role in restricting the pathology of spinocerebellar ataxia type 2 (SCA2). The SCA2 gene produces ataxin-2 proteins which is a cause of familial neurodegenerative diseases. A2BP1 is primarily found in the nucleus but also seen in the cytoplasm and is expressed in the brain, heart, neurons, skeletal muscle, and embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A2BP1 Antibody (NBP2-13169)(3)

Publications using NBP2-13169

• Gu L, Kawaguchi R, Caprioli J, Piri N The effect of Rbfox2 modulation on retinal transcriptome and visual function J Mol Med (Berl) Nov 6 2020 [PMID: 33184471]
• Lund C, Yellapragada V, Vuoristo S et al. Characterization of the human GnRH neuron developmental transcriptome using a GNRH1-TdTomato reporter line in human pluripotent stem cells Dis Model Mech Jan 29 2020 [PMID: 31996360] (PCR, Human)
• Gu L, Bok D, Yu F et al. Downregulation of splicing regulator RBFOX1 compromises visual depth perception. PLoS ONE. Jul 12 2018 [PMID: 30001398] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: RBFOX1
• Entrez:
  • 54715(Human)
  • 268859(Mouse)
• Uniprot:
  • Q9NWB1(Human)
  • Q9JJ43(Mouse)