A2BP1 Antibody (D8H8)

Images

 
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.
Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.
Simple Western: A2BP1 Antibody (D8H8) [NBP2-13169] - Simple Western lane view shows a specific band for A2BP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
D8H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

A2BP1 Antibody (D8H8) Summary

Immunogen
Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
Localization
Nucleus. Cytoplasm. Note: Predominantly nuclear.
Specificity
Does not cross react with paralogue FOX2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RBFOX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
Application Notes
This A2BP1 Antibody (D8H8) is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry on paraffin embedded sections. In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30001398).
Publications
Read Publications using
NBP2-13169 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for A2BP1 Antibody (D8H8)

  • 2BP1
  • A2BP
  • A2BP1 Hexaribonucleotide-binding protein 1
  • Ataxin-2-binding protein 1
  • FOX1 hexaribonucleotide binding protein 1
  • Fox-1 homolog A
  • FOX-1
  • HRNBP1 ataxin 2-binding protein 1
  • RNA binding protein fox-1 homolog 1
  • RNA binding protein, fox-1 homolog (C. elegans) 1

Background

A2BP1, also known as Ataxin-2-binding protein 1, Fox-1 homolog A and Rbfox1, is a member of the Fox-1 family, which is made up of RNA-binding proteins. A2BP1 regulates tissue-specific alternative splicing during erythropoiesis by locating the UGCAUG sequence and binding to the 5'-splice site while also preventing U2AF2 from binding to the 3' splicing site. A2BP1 may play a role in restricting the pathology of spinocerebellar ataxia type 2 (SCA2). The SCA2 gene produces ataxin-2 proteins which is a cause of familial neurodegenerative diseases. A2BP1 is primarily found in the nucleus but also seen in the cytoplasm and is expressed in the brain, heart, neurons, skeletal muscle, and embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for A2BP1 Antibody (NBP2-13169)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for A2BP1 Antibody (NBP2-13169) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-13169AF350
Alexa Fluor 405 NBP2-13169AF405
Alexa Fluor 488 NBP2-13169AF488
Alexa Fluor 532 NBP2-13169AF532
Alexa Fluor 594 NBP2-13169AF594
Alexa Fluor 647 NBP2-13169AF647
Alexa Fluor 700 NBP2-13169AF700
Alexa Fluor 750 NBP2-13169AF750
Biotin NBP2-13169B
DyLight 350 NBP2-13169UV
DyLight 405 NBP2-13169V
DyLight 488 NBP2-13169G
DyLight 550 NBP2-13169R
DyLight 594 NBP2-13169DL594
DyLight 650 NBP2-13169C
DyLight 680 NBP2-13169FR
DyLight 755 NBP2-13169IR
FITC NBP2-13169F
HRP NBP2-13169H
Janelia Fluor 549 NBP2-13169JF549
Janelia Fluor 646 NBP2-13169JF646

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Bioinformatics

Gene Symbol RBFOX1
Entrez
Uniprot