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Nesprin 2 Antibody (6C11)

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Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)
Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform ...read more
Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (6C11) [NBP2-59944] - Immunofluorescence microscopy of HeLa cells. The antibodies can be used on either formaldehyde- or methanol-fixed cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
6C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-59944AF405
Alexa Fluor 488
NBP2-59944AF488
Alexa Fluor 647
NBP2-59944AF647
Alexa Fluor 700
NBP2-59944AF700
Biotin
NBP2-59944B
DyLight 350
NBP2-59944UV
DyLight 405
NBP2-59944V
DyLight 488
NBP2-59944G
DyLight 550
NBP2-59944R
DyLight 650
NBP2-59944C
DyLight 680
NBP2-59944FR
DyLight 755
NBP2-59944IR
FITC
NBP2-59944F
HRP
NBP2-59944H
Janelia Fluor 549
NBP2-59944JF549
Janelia Fluor 646
NBP2-59944JF646
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Nesprin 2 Antibody (6C11) Summary

Immunogen
GST fused to residues 6,653-6,835 of human Nesp2
Specificity
Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SYNE2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1-2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Nesprin 2 Antibody (6C11)

  • DKFZP434H2235
  • DKFZp686E01115
  • DKFZp686H1931
  • EDMD5
  • FLJ46790
  • KIAA1011FLJ11014
  • nesprin 2
  • Nesprin-2
  • NUAFLJ43727
  • NUANCEFLJ45710
  • Nuclear envelope spectrin repeat protein 2
  • Nucleus and actin connecting element protein
  • polytrophin
  • Protein NUANCE
  • spectrin repeat containing, nuclear envelope 2
  • Synaptic nuclear envelope protein 2
  • synaptic nuclei expressed gene 2
  • SYNE-2
  • TROPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SYNE1 Antibody (9F10) [NBP2-59946] - HEK293 cells transiently expressing GFP alone, GFP fused to a small Nesp2 isoform (Nesp2a1) or to a Nesp1 isoform (Nesp1a). The small Nesp1 isoform contains the ~300 residue Nesp1 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from the anti-Nesp1 hybridoma clone (9F10). The antibody is specific for Nesp1.
Immunocytochemistry/Immunofluorescence: SYNE1 Antibody (9F10) [NBP2-59946] - C2C12 cell labeled with the 9F10 monoclonal antibody (green) raised against mouse Nesp1. DNA within the nucleus (blue) is revealed by DAPI-staining.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Emerin Antibody
NBP1-87692
Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum.
Immunohistochemistry: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.
(5 Publications)
Species: Hu, Mu, Rt, Ha
Applications: WB, ICC/IF, IHC, IHC-P, KD
UNC84B Antibody
(3.1E)
NBP2-59942
Western Blot: UNC84B Antibody (3.1E) [NBP2-59942] - Analysis of heart tissue lysates derived from SUN2+/- and SUN2-/- mice.
Immunocytochemistry/Immunofluorescence: UNC84B Antibody (3.1E) [NBP2-59942] - Adult fibroblasts derived from SUN2+/- and SUN2-/- mice. Cells were labeled with the 3.1E monoclonal antibody against SUN2. This antibody can be used on either formaldehyde- or methanol-fixed cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Lamin A + C
Antibody (4C4)
NBP2-25151
Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
Species: Hu
Applications: WB, ICC/IF, IHC
Lamin A Antibody
NBP1-85550
Western Blot: Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human epididymis shows strong nuclear membrane positivity in glandular cells.
Western Blot: Lamin A Antibody [NBP1-85550] - Analysis in human cell line A-549.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
SUN1 Antibody
NBP1-87396
Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows high expression.
Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of A431 cells at 4ug/ml against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.
(9 Publications)
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Neutrophil
Elastase/ELA2 Antibod ...
MAB9167
Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils.
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Meckelin Antibody
NBP1-06590
Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.
Immunohistochemistry-Paraffin: Meckelin Antibody [NBP1-06590] - Staining of kidney tissue section with Meckelin Antibody (NBP1-06590).
(1 Publication)
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MKS1 Antibody
NBP1-88691
Western Blot: MKS1 Antibody [NBP1-88691] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: MKS1 Antibody [NBP1-88691] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
RhoA Antibody
NB100-91273
Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Plectin Antibody
NBP1-84020
Immunocytochemistry/Immunofluorescence: Plectin Antibody [NBP1-84020] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments. Antibody staining is shown in green.
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Species: Hu
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Nesprin-3 Antibody
NBP2-49175
Western Blot: Nesprin-3 Antibody [NBP2-49175] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunohistochemistry: Nesprin-3 Antibody [NBP2-49175] - Staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.
Species: Hu
Applications: WB, IHC, IHC-P
LIS1 Antibody
NBP1-87769
Western Blot: LIS1 Antibody [NBP1-87769] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: LIS1 Antibody [NBP1-87769] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
Immunohistochemistry-Paraffin: LIS1 Antibody [NBP1-87769] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Nucleolin Antibody
NB600-241
Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.
Immunocytochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). This image was submitted via customer Review.
Immunohistochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Verified customer review.
(2 Reviews)
(17 Publications)
Species: Hu, Mu, Rt, Pl
Applications: WB, Simple Western, ChIP, EM, ICC/IF, IHC, IHC-P, IM, ICC
PML Protein
Antibody
NB100-59787
Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500
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gamma-Synuclein
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NBP2-66861
Western Blot: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of gamma Synuclein on rat kidney tissue lysate using anti-gamma Synuclein antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Staining gamma Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of paraffin-embedded rat brain tissue using anti-gamma Synuclein antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Nesprin 2 Antibody
(6C11)
NBP2-59944
Nesprin 2 Antibody (6C11)
Species: Hu
Applications: WB, ICC/IF

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Bioinformatics

Gene Symbol SYNE2