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Nesprin 2 Antibody (6C11)

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Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)
Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform ...read more
Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (6C11) [NBP2-59944] - Immunofluorescence microscopy of HeLa cells. The antibodies can be used on either formaldehyde- or methanol-fixed cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
6C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-59944AF405
Alexa Fluor 488
NBP2-59944AF488
Alexa Fluor 647
NBP2-59944AF647
Alexa Fluor 700
NBP2-59944AF700
Biotin
NBP2-59944B
DyLight 350
NBP2-59944UV
DyLight 405
NBP2-59944V
DyLight 488
NBP2-59944G
DyLight 550
NBP2-59944R
DyLight 650
NBP2-59944C
DyLight 680
NBP2-59944FR
DyLight 755
NBP2-59944IR
FITC
NBP2-59944F
HRP
NBP2-59944H
Janelia Fluor 549
NBP2-59944JF549
Janelia Fluor 646
NBP2-59944JF646
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Nesprin 2 Antibody (6C11) Summary

Immunogen
GST fused to residues 6,653-6,835 of human Nesp2
Specificity
Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SYNE2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1-2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Nesprin 2 Antibody (6C11)

  • DKFZP434H2235
  • DKFZp686E01115
  • DKFZp686H1931
  • EDMD5
  • FLJ46790
  • KIAA1011FLJ11014
  • nesprin 2
  • Nesprin-2
  • NUAFLJ43727
  • NUANCEFLJ45710
  • Nuclear envelope spectrin repeat protein 2
  • Nucleus and actin connecting element protein
  • polytrophin
  • Protein NUANCE
  • spectrin repeat containing, nuclear envelope 2
  • Synaptic nuclear envelope protein 2
  • synaptic nuclei expressed gene 2
  • SYNE-2
  • TROPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: SYNE1 Antibody (JB35-26) [NBP2-75679] - Staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SYNE1 Antibody (JB35-26) [NBP2-75679] - Analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
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Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: UNC84B Antibody (JG39-52) [NBP2-75696] - Staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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NB100-56649
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Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).
Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).
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Western Blot: Lamin A Antibody [NBP1-85550] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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NBP1-87396
Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.
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MKS1 Antibody
NBP1-88691
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Nesprin 2 Antibody
(6C11)
NBP2-59944
Nesprin 2 Antibody (6C11)
Species: Hu
Applications: WB, ICC/IF

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Bioinformatics

Gene Symbol SYNE2