Nesprin 2 Antibody (6C11)

Western Blot: Nesprin 2 Antibody (6C11) [NBP2-59944] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)

Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (6C11) [NBP2-59944] - Immunofluorescence microscopy of HeLa cells. The antibodies can be used on either formaldehyde- or methanol-fixed cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 6C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Nesprin 2 Antibody (6C11) Summary

• Immunogen: GST fused to residues 6,653-6,835 of human Nesp2
• Specificity: Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYNE2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1-2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Nesprin 2 Antibody (6C11)

• DKFZP434H2235
• DKFZp686E01115
• DKFZp686H1931
• EDMD5
• FLJ46790
• KIAA1011FLJ11014
• nesprin 2
• Nesprin-2
• NUAFLJ43727
• NUANCEFLJ45710
• Nuclear envelope spectrin repeat protein 2
• Nucleus and actin connecting element protein
• polytrophin
• Protein NUANCE
• spectrin repeat containing, nuclear envelope 2
• Synaptic nuclear envelope protein 2
• synaptic nuclei expressed gene 2
• SYNE-2
• TROPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNE2