NEK9 Antibody

Immunohistochemistry-Paraffin: NEK9 Antibody [NLS7967] - Anti-NEK9 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK9 Antibody [NLS7967] - Analysis of anti-NEK9 antibody with human brain at 3.6 ug/ml.

Product Discontinued

NEK9 Antibody Summary

• Immunogen: Synthetic 19 amino acid peptide from Pro/Ser/Thr rich domain of human NEK9.
• Localization: Cytoplasmic
• Specificity: Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.6 ug/ml
• Control:

  NEK9 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Monkey (89%), Marmoset (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK9 Antibody

• DKFZp434D0935
• KIAA1995
• MGC138306
• MGC16714
• Nek8
• Nek8NERCC1
• NEK9
• NERCC
• Nercc1 kinase
• NERCCEC 2.7.11.1
• Never in mitosis A-related kinase 9
• NIMA (never in mitosis gene a)- related kinase 9
• NimA-related kinase 8
• NIMA-related kinase Nek8
• NimA-related protein kinase 9
• serine/threonine-protein kinase Nek9

Background

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK9
• Entrez:
  • 91754(Human)