NEK9 Antibody

Immunohistochemistry-Paraffin: NEK9 Antibody [NBP2-31091] - Human Prostate, Carcinoma tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK9 Antibody [NBP2-31091] - IHC analysis of Brain using NBP2-31091 at 4 ug/ ml.

Immunohistochemistry-Paraffin: NEK9 Antibody [NBP2-31091] - Non-Hodgkin's lymphoma.

Immunohistochemistry-Paraffin: NEK9 Antibody [NBP2-31091] - Prostate carcinoma

Immunohistochemistry-Paraffin: NEK9 Antibody [NBP2-31091] - Human Breast, Carcinoma tissue after heat-induced antigen retrieval.

Product Discontinued

NEK9 Antibody Summary

• Immunogen: Synthetic peptide corresponding to the Kinase domain of human NIMA (Never In Mitosis Gene A)-Related Kinase 9 (NEK9)
• Specificity: Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 ug/ml
  • Immunohistochemistry-Paraffin 4 ug/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.7)
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK9 Antibody

• DKFZp434D0935
• KIAA1995
• MGC138306
• MGC16714
• Nek8
• Nek8NERCC1
• NEK9
• NERCC
• Nercc1 kinase
• NERCCEC 2.7.11.1
• Never in mitosis A-related kinase 9
• NIMA (never in mitosis gene a)- related kinase 9
• NimA-related kinase 8
• NIMA-related kinase Nek8
• NimA-related protein kinase 9
• serine/threonine-protein kinase Nek9

Background

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEK9 Antibody (NBP2-31091)(2)

Publications using NBP2-31091

• Lu G, Du R, Dong J et al. Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9 Cell death & disease 2023-07-13 [PMID: 37443302] (IHC, Human)
• Lu G, Tian S, Sun Y, et al. NEK9, a novel effector of IL-6/STAT3, regulates metastasis of gastric cancer by targeting ARHGEF2 phosphorylation Theranostics 2021-01-01 [PMID: 33500736] (IHC-P, Human)

Bioinformatics

• Gene Symbol: NEK9
• Uniprot:
  • NP_149107(Human)