NEK3 Antibody - BSA Free

Western Blot: NEK3 Antibody [NBP1-32624] - Sample (30ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NEK3 Antibody [NBP1-32624] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NEK3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NEK3 Antibody - BSA Free (NBP1-32624) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NEK3. The exact sequence is proprietary.
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NEK3 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• glycogen synthase A kinase
• HSPK 36
• HSPK36
• hydroxyalkyl-protein kinase
• MGC29949
• Never in mitosis A-related kinase 3
• NIMA (never in mitosis gene a)-related kinase 3
• NimA-related protein kinase 3
• phosphorylase B kinase kinase
• serine/threonine-protein kinase Nek3

Background

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NEK3