NEK3 Antibody

Images

 
Western Blot: NEK3 Antibody [NBP1-32624] - Sample (30ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NEK3 Antibody [NBP1-32624] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

NEK3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NEK3. The exact sequence is proprietary.
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NEK3 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • glycogen synthase A kinase
  • HSPK 36
  • HSPK36
  • hydroxyalkyl-protein kinase
  • MGC29949
  • Never in mitosis A-related kinase 3
  • NIMA (never in mitosis gene a)-related kinase 3
  • NimA-related protein kinase 3
  • phosphorylase B kinase kinase
  • serine/threonine-protein kinase Nek3

Background

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEK3