NEK2 Antibody (1B6) - Azide and BSA Free

Western Blot: NEK2 Antibody (1B6) [NBP1-41207] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (NBP1-41207) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu
• Applications: WB
• Clone: 1B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NEK2 Antibody (1B6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NEK2 Antibody (1B6) - Azide and BSA Free (NBP1-41207) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide Immunogen sequence information is commercially sensitive
• Localization: Nuclear. Localizes at the centromere. Isoform 1 has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end missing in isoform 2 which is predominantly cytoplasmic.
• Specificity: This antibody will recognize human NEK2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEK2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: WB: Detects both isoforms 1 and 2 in Jurkat lysate.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for NEK2 Antibody (1B6) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• HSPK 21
• HsPK21
• NEK2
• NEK2AHsPK21
• Never in mitosis A-related kinase 2
• NIMA (never in mitosis gene a)-related kinase 2
• NimA-like protein kinase 1
• NimA-related protein kinase 2
• NLK1
• NLK1serine/threonine-protein kinase Nek2

Background

NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.Both isoforms are expressed in pheripheral T cells and many other transformed cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK2
• Entrez:
  • 4751(Human)