NEIL2 Overexpression Lysate (Native)

Images

 
Western Blot: NEIL2 Overexpression Lysate (Adult Normal) [NBP2-09579] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEIL2.

Product Details

Summary
Applications WB

Order Details

NEIL2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001129218. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens NEIL2, mRNA.
Gene
NEIL2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NEIL2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NEIL2 Overexpression Lysate (Native)

  • DNA glycosylase/AP lyase Neil2
  • EC 3.2.2.-
  • EC 4.2.99.18
  • endonuclease 8-like 2
  • Endonuclease VIII-like 2
  • FLJ31644
  • MGC2832
  • MGC4505
  • NEH2DNA-(apurinic or apyrimidinic site) lyase Neil2
  • nei endonuclease VIII-like 2 (E. coli)
  • Nei homolog 2
  • nei like 2 (E. coli)
  • nei like 2
  • NEI2
  • Nei-like protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NBP2-47358
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-106
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-108
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-441
Species: Hu
Applications: WB
NB600-1025
Species: Hu, Mu, Rt, Bv, Ha, Xp
Applications: WB, IHC, IHC-P, IP
NB600-1031
Species: Hu, Mu, Rt, Rb
Applications: WB, ICC/IF, IHC-P
NBP1-87257
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-32822
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP
NBP2-02280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88284
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NBP1-97572
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
NB600-1032
Species: Hu, Mu, Rt, Ba
Applications: WB, ICC/IF, IHC
AF1379
Species: Hu
Applications: WB, IHC
AF1067
Species: Hu, Mu, Rt
Applications: WB, IHC, Neut
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-09579
Applications: WB

Publications for NEIL2 Lysate (NBP2-09579) (0)

There are no publications for NEIL2 Lysate (NBP2-09579).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NEIL2 Lysate (NBP2-09579) (0)

There are no reviews for NEIL2 Lysate (NBP2-09579). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NEIL2 Lysate (NBP2-09579) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NEIL2 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for NEIL2 Lysate (NBP2-09579)

Discover related pathways, diseases and genes to NEIL2 Lysate (NBP2-09579). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NEIL2 Lysate (NBP2-09579)

Discover more about diseases related to NEIL2 Lysate (NBP2-09579).
 

Pathways for NEIL2 Lysate (NBP2-09579)

View related products by pathway.

PTMs for NEIL2 Lysate (NBP2-09579)

Learn more about PTMs related to NEIL2 Lysate (NBP2-09579).
 

Research Areas for NEIL2 Lysate (NBP2-09579)

Find related products by research area.

Blogs on NEIL2

There are no specific blogs for NEIL2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NEIL2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol NEIL2

Customers Who Bought This Also Bought

OGG1 Antibody
NB100-106
OGG1 Antibody
NB100-106
OGG1 Antibody
NB100-106
NEIL2 Antibody
NBP2-55727
NEIL2 Antibody
NBP2-55727