NEDP1/SENP8 Antibody - BSA Free

Western Blot: NEDP1/SENP8 Antibody [NBP1-31208] - Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NEDP1/SENP8 Antibody [NBP1-31208] - SW480 xenograft, using SENP8 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NEDP1/SENP8 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NEDP1/SENP8. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NEDP1/SENP8 Antibody - BSA Free

• DEN1
• DEN1deneddylase-1
• deneddylase 1
• Deneddylase-1
• EC 3.4.22.-
• HsT17512
• NEDD8-specific protease 1
• NEDP1
• NEDP1NEDD8 specific-protease cysteine 2
• Protease, cysteine 2
• protease, cysteine, 2 (NEDD8 specific)
• PRSC2
• SENP8
• Sentrin/SUMO-specific protease SENP8
• sentrin-specific protease 8
• SUMO sentrin specific protease family member 8
• SUMO/sentrin specific peptidase family member 8
• SUMO/sentrin specific protease family member 8

Background

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SENP8