NEDD8 Antibody (584606)

HAF007). A specific band was detected for NEDD8 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." > HAF007). A specific band was detected for NEDD8 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." title="Western blot shows lysates of Raji human Burkitt's lymphoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB4936) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEDD8 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." />

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB4936) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 584606
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NEDD8 Antibody (584606) Summary

• Immunogen: E. coli-derived recombinant human NEDD8
  Leu2-Gly77
  Accession # Q15843
• Specificity: Detects human, mouse, and rat NEDD8 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEDD8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEDD8 Antibody (584606)

• FLJ43224
• MGC104393
• MGC125896
• MGC125897
• NEDD8
• Nedd-8
• Neddylin
• Neural precursor cell expressed developmentally down-regulated protein 8
• neural precursor cell expressed, developmentally down-regulated 8
• Rub1
• Ubiquitin-like protein Nedd8

Background

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6‑8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81 amino acids (aa) in length and contains one ubiquitin-like domain (aa 1‑65), and a 4‑5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEDD8 Antibody (MAB4936)(1)

Publication using MAB4936

• Pang, EZ;Zhao, B;Flowers, C;Oroudjeva, E;Winter, JB;Pandey, V;Sawaya, MR;Wohlschlegel, J;Loo, JA;Rodriguez, JA;Clarke, SG; Structural basis for L-isoaspartyl-containing protein recognition by the human PCMTD1 cullin-RING E3 ubiquitin ligase The Journal of biological chemistry 2025-11-01 [PMID: 40975169] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NEDD8
• Uniprot:
  • Q15843(Human)