NEDD8 Activating Enzyme (APPBP1/UBA3) Overexpression Lysate (Native)

Images

 
Western Blot: NAE1 Overexpression Lysate (Adult Normal) [NBP2-09795] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAE1.

Product Details

Summary
Applications WB

Order Details

NEDD8 Activating Enzyme (APPBP1/UBA3) Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_003896. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens NAE1, mRNA.
Gene
NAE1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
60.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for NEDD8 Activating Enzyme (APPBP1/UBA3)

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NEDD8 Activating Enzyme (APPBP1/UBA3) Overexpression Lysate (Native)

  • A-116A10.1
  • amyloid beta precursor protein binding protein 1
  • amyloid beta precursor protein binding protein 1, 59kDa
  • Amyloid beta precursor protein-binding protein 1, 59 kDa
  • amyloid beta precursor protein-binding protein 1, 59kD
  • Amyloid protein-binding protein 1
  • APPBP1APP-BP1
  • NEDD8 Activating Enzyme (APPBP1/UBA3)
  • NEDD8 activating enzyme E1 subunit 1
  • NEDD8-activating enzyme E1 regulatory subunit
  • NEDD8-activating enzyme E1 subunit
  • protooncogene protein 1
  • Proto-oncogene protein 1
  • ula-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-01863
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-83150
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56552
Species: Hu
Applications: WB
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
NB100-91724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, ICC
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-19957
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB3765
Species: Hu, Mu
Applications: WB, Simple Western, ICC, ICFlow
NBP1-82454
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-41182
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-54465
Species: Hu, Mu, Rt
Applications: IHC (-), WB, IP
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, IF

Publications for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795) (0)

There are no publications for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795) (0)

There are no reviews for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NEDD8 Activating Enzyme (APPBP1/UBA3) Products

Related Products by Gene

Bioinformatics Tool for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795)

Discover related pathways, diseases and genes to NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795)

Discover more about diseases related to NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795).
 

Pathways for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795)

View related products by pathway.

Research Areas for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBP2-09795)

Find related products by research area.

Blogs on NEDD8 Activating Enzyme (APPBP1/UBA3)

There are no specific blogs for NEDD8 Activating Enzyme (APPBP1/UBA3), but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol NAE1
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-09795 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106
p19ARF Antibody
NB200-106