NEDD4L Antibody (3W9J2)

Western Blot: NEDD4L Antibody (3W9J2) [NBP3-16747] - Western blot analysis of extracts of various cell lines, using NEDD4L antibody (NBP3-16747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

• Reactivity: Hu
• Applications: WB
• Clone: 3W9J2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NEDD4L Antibody (3W9J2) Summary

• Description: Novus Biologicals Rabbit NEDD4L Antibody (3W9J2) (NBP3-16747) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 876-975 of human NEDD4L (Q96PU5). NHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NEDD4L
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NEDD4L Antibody (3W9J2)

• E3 ubiquitin-protein ligase NEDD4-like
• EC 6.3.2
• EC 6.3.2.-
• FLJ33870
• hNedd4-2
• KIAA0439ubiquitin-protein ligase NEDD4-like
• NEDD4.2
• NEDD4-2
• NEDL3
• neural precursor cell expressed, developmentally down-regulated 4-like
• neural precursor cell expressed, developmentally down-regulated gene 4-like
• RSP5ubiquitin-protein ligase Rsp5

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEDD4L