NEDD1 Antibody

Western Blot: NEDD1 Antibody [NB100-55420] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NEDD1 Antibody Summary

• Immunogen: Peptide with sequence C-NQTRNSEKFEKPEN corresponding to internal region according to NP_690869.1.
• Epitope: NQTRNSEKFEKPEN
• Marker: Centrosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEDD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in HeLa lysates (calculated MW of 72.0 kDa band according to NP_690869.1). An additional band was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide and likely represents the mature form.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEDD1 Antibody

• FLJ35902
• GCP-WD
• NEDD-1
• Neural precursor cell expressed developmentally down-regulated protein 1
• neural precursor cell expressed, developmentally down-regulated 1
• protein NEDD1
• TUBGCP7

Background

May play an important role during the embryonic development and differentiation of the central nervous system. Human NEDD1 localises to the centrosome and mitotic spindle. It binds to the components of the gamma-tubulin ring complex and target this complex to the centrosome and spindle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEDD1 Antibody (NB100-55420)(2)

Publications using NB100-55420

• Roubin R, Acquaviva C, Chevrier V et al. Myomegalin is necessary for the formation of centrosomal and Golgi-derived microtubules. Biol Open 2013-02-15 [PMID: 23430395]
• Haren L, Remy MH, Bazin I et al. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol 2006-02-13 [PMID: 16461362]

Bioinformatics

• Gene Symbol: NEDD1
• Entrez:
  • 121441(Human)
• Uniprot:
  • NP_690869.1(Human)