Nectin-3/PVRL3 Antibody - BSA Free

Staining of human cell line U-251 MG shows localization to microtubule organizing center.

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody [NBP1-86553] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody [NBP1-86553] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody [NBP1-86553] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody [NBP1-86553] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Nectin-3/PVRL3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSF
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NECTIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Nectin-3/PVRL3 Protein (NBP1-86553PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22904683).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Nectin-3/PVRL3 Antibody - BSA Free

• CD113 antigen
• CD113
• CDw113FLJ90624
• DKFZp566B0846
• nectin 3
• Nectin3
• Nectin-3
• poliovirus receptor-related 3
• poliovirus receptor-related protein 3
• PPR3
• PRR3CD113
• PVRL3
• PVRR3

Background

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-3/PVRL3 Antibody (NBP1-86553)(2)

Publications using NBP1-86553

• Haeberle H, Dudley JT, Liu JT et al. Identification of Cell Surface Targets through Meta-analysis of Microarray Data. Neoplasia. 2012-07-01 [PMID: 22904683] (IF/IHC, Human)
• Bolander A, Agnarsdottir M, Stromberg S et al. The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas. Cancer Genomics Proteomics 2008-11-01 [PMID: 19287070]

Bioinformatics

• Gene Symbol: NECTIN3