Nectin-2/CD112 Antibody (610603) [Allophycocyanin]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Nectin-2/CD112 APC-conjugated Monoclonal Antibody (Catalog # FAB2229A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 610603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Nectin-2/CD112 Antibody (610603) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-2/CD112 isoform a
  Gln32-Leu360
  Accession # NP_002847
• Specificity: Detects human Nectin-2/CD112 by flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PVRL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-2/CD112 Antibody (610603) [Allophycocyanin]

• CD112 antigen
• CD112
• Herpes virus entry mediator B
• Herpesvirus entry mediator B
• herpesvirus entry protein B
• HVEB
• HVEBpoliovirus receptor-like 2
• MPH
• nectin 2
• Nectin2
• Nectin-2
• poliovirus receptor-related 2 (herpesvirus entry mediator B)
• poliovirus receptor-related protein 2
• PRR2
• PRR2nectin-2
• PVRL2
• PVRR2poliovirus receptor related 2

Background

Nectins are a small family of Ca⁺⁺-independent Immunoglobulin (Ig)-like Cell Adhesion Molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types (2, 3). It has two splice forms (4, 5). Nectin-2δ is a 65 kDa long form and is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular region, a 21 aa TM segment, and a 157 aa cytoplasmic domain. The extracellular region contains one N-terminal 85 aa V-type Ig domain and two 45-55 aa C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (6). The short, 60 kDa isoform of Nectin-2 (Nectin-2 alpha ) has the same signal sequence and extracellular domain as nectin-2δ, but differs in the TM and cytoplasmic region (4, 5). In this case, the cytoplasmic tail is only 94 aa in length. The human extracellular region shows 72% aa sequence identity with the equivalent region in mouse. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.

1. Takai, Y. and H. Nakanishi, 2003, J. Cell Sci. 116:17.
2. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
3. Pende, D. et al. (2005) Mol. Immunol. 42:463.
4. Eberle, F. et al. (1995) Gene 159:267.
5. Warner, M.S. et al. (1998) Virology 246:179.
6. Struyf, F. et al. (2002) J. Virol. 76:12940.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-2/CD112 Antibody (FAB2229A)(6)

Publications using FAB2229A

• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human)
• Bérengère Salomé, John P. Sfakianos, Daniel Ranti, Jorge Daza, Christine Bieber, Andrew Charap et al. NKG2A and HLA-E define an alternative immune checkpoint axis in bladder cancer Cancer Cell 2022-09-01 [PMID: 36099881]
• S Battella, S Oliva, L Franchitti, R La Scaleia, A Soriani, S Isoldi, C Capuano, C Pighi, S Morrone, R Galandrini, A Santoni, G Palmieri Fine tuning of the DNAM-1/TIGIT/ligand axis in mucosal T cells and its dysregulation in pediatric inflammatory bowel diseases (IBD) Mucosal Immunol, 2019-10-03;0(0):. 2019-10-03 [PMID: 31582819] (Flow Cytometry, Human)
• R Molfetta, ND Milito, B Zitti, M Lecce, C Fionda, M Cippitelli, A Santoni, R Paolini The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing Eur. J. Immunol., 2019-03-27;0(0):. 2019-03-27 [PMID: 30888046] (Flow Cytometry, Human)
• B Zitti, R Molfetta, C Fionda, L Quatrini, H Stabile, M Lecce, V de Turris, MR Ricciardi, MT Petrucci, M Cippitelli, A Gismondi, A Santoni, R Paolini Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells Sci Rep, 2017-09-05;7(1):10445. 2017-09-05 [PMID: 28874810] (Flow Cytometry, Human)
• N Josifovska, DJ Szabó, R Nagymihály, Z Veréb, A Facskó, K Eriksen, MC Moe, G Petrovski Cultivation and characterization of pterygium as an ex vivo study model for disease and therapy Cont Lens Anterior Eye, 2017-05-24;0(0):. 2017-05-24 [PMID: 28550976] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PVRL2
• Uniprot:
  • NP_002847()