NECAB1 Antibody (OTI4G8)

Western Blot: NECAB1 Antibody (4G8) [NBP2-00480] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NECAB1 antibody, and then analyzed by flow cytometry.

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - Analysis of Hela cells, using anti-NECAB1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - Analysis of Jurkat cells, using anti-NECAB1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow
• Clone: OTI4G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.59 mg/ml

NECAB1 Antibody (OTI4G8) Summary

• Description: Novus Biologicals Mouse NECAB1 Antibody (OTI4G8) (NBP2-00480) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECAB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:2000
• Theoretical MW: 40.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.59 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NECAB1 Antibody (OTI4G8)

• EF hand calcium binding protein 1
• EFCBP1
• EF-hand calcium binding protein 1
• EF-hand calcium-binding protein 1
• neuronal calcium binding protein
• Neuronal calcium-binding protein 1
• N-terminal EF-hand calcium binding protein 1
• N-terminal EF-hand calcium-binding protein 1
• STIP-1
• synaptotagmin interacting protein 1

Background

The NECAB1 gene codes for a N-terminal EF-hand calcium-binding protein 1 that in isoform 1 is 351 amino acids long and 40 kDA and in isoform 2 is 100 amino acids long at 11 kDA. NECAB1 has been linked to diseases such as seizures, schizophrenia, alzheimer's disease, motor neuron disease, neuronitis, and neurodegenerative disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECAB1
• Entrez:
  • 64168(Human)