Nebulin Antibody (1F4) - Azide and BSA Free

Sandwich ELISA: Nebulin Antibody (1F4) [H00004703-M01] - Detection limit for recombinant GST tagged NEB is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nebulin Antibody (1F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NEB (NP_004534, 2108 a.a. ~ 2217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NTKTSYHTPADMLSVTAAKDAQANITNTNYKHLIHKYILLPDAMNIELTRNMNRIQSDNEYKQDYNEWYKGLGWSPAGSLEVEKAKKATEYASDQKYRQHPSNFQFKKLT
• Specificity: NEB (1F4)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nebulin Antibody (1F4) - Azide and BSA Free

• DKFZp686C1456
• FLJ11505
• FLJ36536
• FLJ39568
• FLJ39584
• NEB177D
• nebulin
• NEM2

Background

This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-,and developmental stage-specific. The 6,669-residue predicted protein, the short form, contains 185 copies of 35-amino acid modules that can be classified into 7 types. It is suggested that alternative splicing may explain the developmental or tissue-specific size variants of nebulin. However, the nucleotide sequence information is not available for other spliced products. Mutations in this gene are associated with recessive nemaline myopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEB
• Entrez:
  • 4703(Human)
• OMIM:
  • 161650(Human)
• Uniprot:
  • NP_004534(Human)