NDUFS7 Antibody

Images

 
Western Blot: NDUFS7 Antibody [NBP1-49846] - (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDUFS7 Antibody [NBP1-49846] - Staining of paraffin embedded Human Liver at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: NDUFS7 Antibody [NBP1-49846] - Staining of paraffin embedded Human Cortex at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NDUFS7 Antibody Summary

Immunogen
Peptide with sequence C-SRGEYVVAKLD corresponding to internal region according to NP_077718.3.
Epitope
SRGEYVVAKLD - internal region
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NDUFS7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 20 kDa band observed in human heart and human skeletal muscle lysates (calculated MW of 23.6 kDa band according to NP_077718.3).
Publications
Read Publication using NBP1-49846.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NDUFS7 Antibody

  • CI-20
  • CI-20kD
  • complex I, mitochondrial respiratory chain, 20-KD subunit
  • Complex I-20kD
  • EC 1.6.5.3
  • EC 1.6.99.3
  • EC 1.6.99.5
  • FLJ45860
  • FLJ46880
  • MGC120002
  • MY017
  • NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Qreductase)
  • NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • NADH:ubiquinone oxidoreductase PSST subunit
  • NADH-coenzyme Q reductase
  • NADH-ubiquinone oxidoreductase 20 kDa subunit
  • PSST subunit
  • PSSTcomplex I 20kDa subunit

Background

This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS7 Antibody (NBP1-49846)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFS7
Entrez
Uniprot