NDUFS7 Antibody

Western Blot: NDUFS7 Antibody [NBP1-49846] - (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NDUFS7 Antibody [NBP1-49846] - Staining of paraffin embedded Human Liver at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: NDUFS7 Antibody [NBP1-49846] - Staining of paraffin embedded Human Cortex at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS7 Antibody Summary

• Description: Novus Biologicals Goat NDUFS7 Antibody (NBP1-49846) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NDUFS7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SRGEYVVAKLD corresponding to internal region according to NP_077718.3.
• Epitope: SRGEYVVAKLD - internal region
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human heart and human skeletal muscle lysates (calculated MW of 23.6 kDa band according to NP_077718.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS7 Antibody

• CI-20
• CI-20kD
• complex I, mitochondrial respiratory chain, 20-KD subunit
• Complex I-20kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• FLJ45860
• FLJ46880
• MGC120002
• MY017
• NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
• NADH:ubiquinone oxidoreductase PSST subunit
• NADH-coenzyme Q reductase
• NADH-ubiquinone oxidoreductase 20 kDa subunit
• PSST subunit
• PSSTcomplex I 20kDa subunit

Background

This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS7 Antibody (NBP1-49846)(1)

Publication using NBP1-49846

• Starr JM, Shiels PG, Harris SE et al. Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Mech Ageing Dev 2008-12-01 [PMID: 18977241]

Bioinformatics

• Gene Symbol: NDUFS7
• Entrez:
  • 374291(Human)
  • 75406(Mouse)
  • 362837(Rat)
• Uniprot:
  • NP_077718.3(Human)