NDUFB8 Antibody (JG61-36)

Western Blot: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: A549 Lane 3: Mouse heart Lane 4: Rat spleen

Immunohistochemistry-Paraffin: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Flow Cytometry: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NDUFB8 Antibody (JG61-36) [NBP2-75586] - Analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Product Discontinued

NDUFB8 Antibody (JG61-36) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human NDUFB8 aa 1-186 / 186. (SwissProt: O95169 Human; SwissProt: Q9D6J5 Mouse)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDUFB8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NDUFB8 Antibody (JG61-36)

• CI-ASHImitochondrial
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• NADH:ubiquinone oxidoreductase ASHI subunit
• NADH-ubiquinone oxidoreductase ASHI subunit

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFB8 Antibody (NBP2-75586)(3)

Publications using NBP2-75586

• Zuliani I, Lanzillotta C, Tramutola A et al. High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimers Disease Signatures International Journal of Molecular Sciences 2021-04-03 [PMID: 33916835] (WB, IP, Mouse)
• Wang YP, Sharda A, Xu SN et al. Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis Cell metabolism 2021-03-23 [PMID: 33770508] (WB)
• Zuliani I, Urbinati C, et al. The Anti-Diabetic Drug Metformin Rescues Aberrant Mitochondrial Activity and Restrains Oxidative Stress in a Female Mouse Model of Rett Syndrome. J Clin Med 2020-06-01 [PMID: 32492904] (WB, Mouse)

Bioinformatics

• Gene Symbol: NDUFB8