NDUFB7 Antibody (4D4)

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 expression in transfected 293T cell line by NDUFB7 monoclonal antibody (M01), clone 4D4.Lane 1: NDUFB7 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 over-expressed 293 cell line, cotransfected with NDUFB7 Validated Chimera RNAi ( Cat # H00004713-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFB7 monoclonal antibody (M01), clone 4D4 (Cat # H00004713-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - NDUFB7 monoclonal antibody (M01), clone 4D4 Analysis of NDUFB7 expression in A-431.

ELISA: NDUFB7 Antibody (4D4) [H00004713-M01] - Detection limit for recombinant GST tagged NDUFB7 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 4D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NDUFB7 Antibody (4D4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NDUFB7 (NP_004137, 38 a.a. ~ 137 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EMVATQQEMMDAQLRLQLRDYCAHHLIRLLKCKRDSFPNFLACKQERHDWDYCEHRDYVMRMKEFERERRLLQRKKRREKKAAELAKGQGPGEVDPKVAL
• Specificity: NDUFB7 (4D4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDUFB7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDUFB7 Antibody (4D4)

• B18
• Cell adhesion protein SQM1
• CI-B18NADH-ubiquinone oxidoreductase B18 subunit
• complex I B18 subunit
• Complex I-B18
• MGC2480
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (18kD, B18)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Background

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB7
• Entrez:
  • 4713(Human)
• OMIM:
  • 603842(Human)
• Uniprot:
  • NP_004137(Human)