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NDUFB5 Antibody

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Western Blot: NDUFB5 Antibody [NBP2-95246] - Analysis of extracts of various cell lines, using NDUFB5 .Exposure time: 10s.
Western Blot: NDUFB5 Antibody [NBP2-95246] - Analysis of extracts of various cell lines, using NDUFB5 .Exposure time: 10s.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NDUFB5 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFB5
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NDUFB5 Antibody

  • 5 (16kD, SGDH)
  • CISGDH
  • CI-SGDH
  • Complex I-SGDH
  • DKFZp686N02262
  • FLJ30597
  • MGC111204
  • MGC12314
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • SGDH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: AHNAK Antibody (3G7) [H00079026-M01] - Detection limit for recombinant GST tagged AHNAK is approximately 0.03ng/ml as a capture antibody.
Immunoprecipitation: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK transfected lysate using anti-AHNAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHNAK MaxPab rabbit polyclonal antibody.
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Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
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Western Blot: SLC25A12 Antibody [NBP1-89020] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Western Blot: SLC25A12 Antibody [NBP1-89020] - Analysis using Anti-SLC25A12 antibody NBP1-89020 (A) shows similar pattern to independent antibody NBP2-38249 (B).
Immunohistochemistry-Paraffin: SLC25A12 Antibody [NBP1-89020] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
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Western Blot: HBLD2 Antibody [NBP1-89715] - Analysis in control (vector only transfected HEK293T lysate) and ISCA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HBLD2 Antibody [NBP1-89715] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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Immunocytochemistry/Immunofluorescence: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of monoclonal antibody to FMN1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of FMN1 expression in human colon.
Species: Hu
Applications: ELISA, ICC/IF, WB
SDHB Antibody
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Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Staining of human liver shows moderate to strong granular cytoplasmic postivity in hepatocytes.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
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Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
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Aminopeptidase O/ONPEP Antibody
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Applications: IHC, IP, WB
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Western Blot: UQCRB Antibody [NBP3-12711] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa
Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human kidney with rabbit polyclonal antibody at 1:5000 dilution.
Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution.
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Applications: IHC, IHC-P, IP, WB
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Western Blot: COX5A Antibody [NBP1-32550] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: COX5A Antibody [NBP1-32550] - Sample(30 ug whole cell lysate)A:HeLa S3 B:Hep G2 15% SDS PAGE, antibody diluted at 1:5000.
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ATP5G3 GST (N-Term) P ...
H00000518-P01
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Immunocytochemistry/Immunofluorescence: NDUFB4 Antibody [NBP3-03538] - Analysis of L929 cells using NDUFB4 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunocytochemistry/Immunofluorescence: NDUFB3 Antibody [NBP2-93366] - Analysis of L929 cells using NDUFB3 . Blue: DAPI for nuclear staining.
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Western Blot: Ndufs1 Antibody [NBP1-56520] - Nuclear lysates at 1:1000.
Western Blot: Ndufs1 Antibody [NBP1-56520] - Lanes: Lane1 : 30 ug Human liver Lane2: 30 ug Rat liver Lane3: 30 ug Mouse (wild-type) liver Lane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liver Lane5: 30 ug Human muscle Lane6: 30 ug Rat muscle Lane7: 30 ug Mouse muscle Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Secondary, Antibody Dilution: 1 : 10000 Gene name: NDUFS1.
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Antibody (242305) [Un ...
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Western Blot: KAT6B-MORF Antibody [NBP3-02990] - Analysis of extracts of various cell lines, using KAT6B-MORF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit
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Knockout Validated: NDUFV1 Antibody [NBP1-33074] - Wild-type (WT) and NDUFV1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Paraffin-embedded mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Paraffin-embedded mouse stomach. NDUFV1 antibody [N3C3] diluted at 1:500.
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Bioinformatics

Gene Symbol NDUFB5