NDUFB5 Antibody - BSA Free

Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.

Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-31684] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: NDUFB5 Antibody [NBP1-31684] - 50 ug mouse brain lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684] - NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NDUFB5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC, IHC-Fr (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFB5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NDUFB5 Antibody - BSA Free (NBP1-31684) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NDUFB5. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane, Single-pass membrane protein, Matrix side
• Predicted Species: Monkey (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFB5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%) and Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NDUFB5 Antibody - BSA Free

• 5 (16kD, SGDH)
• CISGDH
• CI-SGDH
• Complex I-SGDH
• DKFZp686N02262
• FLJ30597
• MGC111204
• MGC12314
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
• SGDH

Background

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFB5