NDUFB4 Antibody

Western Blot: NDUFB4 Antibody [NBP2-15035] - Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry: NDUFB4 Antibody [NBP2-15035] - NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. NDUFB4 antibody (NBP2-15035) dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NDUFB4 Antibody Summary

• Description: Novus Biologicals Rabbit NDUFB4 Antibody (NBP2-15035) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human NDUFB4 Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFB4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NDUFB4 Antibody

• B15
• CI-B15
• complex I B15 subunit
• Complex I-B15
• MGC5105
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
• NADH-ubiquinone oxidoreductase B15 subunit

Background

The protein encoded by this gene belongs to the complex I NDUFB4 subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFB4
• Uniprot:
  • O95168(Human)