NCOA4 Antibody - BSA Free

Western Blot: NCOA4 Antibody [NBP1-28720] - Detection of human ARA70 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody NBP1-28720 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: NCOA4 Antibody [NBP1-28720] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA4 antibody NBP1-28720 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCOA4 was also immunoprecipitated by rabbit anti-NCOA4 antibody NBP1-28681, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NCOA4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NCOA4 Antibody - BSA Free (NBP1-28720) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 564 and 614 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA4 Antibody - BSA Free

• Androgen receptor coactivator 70 kDa protein
• Androgen receptor-associated protein of 70 kDa
• ARA70RFGret fused
• DKFZp762E1112
• ELE1NCoA-4
• nuclear receptor coactivator 4,70 kDa AR-activator
• PTC3RET-activating gene ELE1
• Ret-activating protein ELE1,70 kDa androgen receptor coactivator

Background

ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA4
• Entrez:
  • 8031(Human)
• Uniprot:
  • NP_005428.1(Human)